BioProject

Candida albicans is part of the human gastrointestinal (GI) microbiota. To better understand how C. More...

Candida albicans is part of the human gastrointestinal (GI) microbiota. To better understand how C. albicans efficiently establishes GI colonisation, we competitively challenged growth of 572 signature-tagged strains (~10% genome coverage), each conditionally overexpressing a single gene, in the murine gut. We identified CRZ2, a transcription factor whose overexpression and deletion respectively increased and decreased early GI colonisation. Using clues from genome-wide expression and gene-set enrichment analyses, we found that the optimal activity of Crz2p occurs under hypoxia at 37°C, as evidenced by both phenotypic and transcriptomic analyses following CRZ2 genetic perturbation. Consistent with early colonisation of the GI tract, we show that CRZ2 overexpression confers resistance to acidic pH and bile salts, suggesting an adaptation to the upper sections of the gut. Genome-wide location analyses revealed that Crz2p directly modulates the expression of many mannosyltransferase- and cell-wall protein-encoding genes, suggesting a link with cell-wall function. We show that CRZ2 overexpression alters cell-wall phosphomannan abundance and increases sensitivity to tunicamycin, suggesting a role in protein glycosylation. Our study reflects the powerful use of gene overexpression as a complementary approach to gene deletion to identify relevant biological pathways involved in C. albicans interaction with the host environment. Overall design: Two-condition experiments: 1) Samples 1-6: Comparison of Candida albicans TETp-CRZ2 strain treated (+Dox, Cy5-labeled) or untreated (-Dox, Cy3-labeled) with 40 µg/mL of doxycyline during 2h and 4h in Yeast Peptone Dextrose medium, 30ºC, 3 biological replicates for each time-point. 2) Samples 7-12: Comparison of a Candida albicans wild-type strain (Cy3-labeled) to a crz2-/- mutant (Cy5-labeled) grown under normoxia at 30ºC (standard lab growth conditions, Samples 7-9) or under hypoxia at 37ºC (BBL GasPak anaerobic jar in an incubator at 37ºC, Samples 10-12). 3) Samples 13-18: Comparison of Normoxia 30ºC-grown (Norm30C, Cy3-labeled) wild-type or crz2-/- mutant strains to hypoxia 37ºC-grown (Hyp37C, Cy5-labeled) wild-type (Samples 13-15) or crz2-/- mutant (Samples 16-18) strains, respectively. 4) Samples 19-22: Comparison of Candida albicans TETp-only (empty vector) strain treated (+Dox, Cy5-labeled) or untreated (-Dox, Cy3-labeled) with 40 µg/mL of doxycyline during 4h in Yeast Peptone Dextrose medium, 30ºC, 4 biological replicates. Less...