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Accession: PRJNA279169 ID: 279169

Mus musculus (house mouse)

Precocious Interleukin 21 expression in naïve mice defines a novel stage of T-follicular helper cell development

See Genome Information for Mus musculus
To define early forming IL21-expressing CD4+ T cells at the transcriptional level, we performed RNAseq on FACS-purified splenic CD4+ T cells from naïve 4 wk old IL21-VFP reporter mice based on the following criteria: naïve ICOSlo/- VFP- (N); activated ICOShi VFP- cells not expressing IL21 (ACT); and activated IL21-expressing cells ICOShi VFP+ cells (ACT IL21) Overall design: mRNA profiles of FACS-purified VFP-ICOS-, VFP-ICOS+, and VFP+ICOS+ CD4+ cell pools from naïve 4 wk old IL21-VFP reporter mice were generated by deep sequencing, in duplicate, using Illumina MiSeq
AccessionPRJNA279169; GEO: GSE67171
Data TypeTranscriptome or Gene expression
ScopeMultiisolate
OrganismMus musculus[Taxonomy ID: 10090]
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus; Mus musculus
SubmissionRegistration date: 23-Mar-2015
The Jackson Laboratory
RelevanceModel Organism
Project Data:
Resource NameNumber
of Links
Sequence data
SRA Experiments6
Other datasets
BioSample6
GEO DataSets1
GEO Data Details
ParameterValue
Data volume, Supplementary Mbytes1
SRA Data Details
ParameterValue
Data volume, Gbases42
Data volume, Mbytes27672

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