Thermophilic viruses (phages) were first reported decades ago; however, our knowledge of their diversity, biology and ecological significance is still very limited. Previous research on thermophilic viruses has focused on cultured viruses. This study examined the genomic profiles and morphological diversity of uncultured viruses directly isolated from 74° to 93°C alkaline hot springs in Yellowstone National Park. Viral abundance ranged from 1E5 to 1E6 particles per milliliter, and a variety of bacteriophage and crenarchaeal virus-like morphologies were observed. Using a new method for constructing metagenomic libraries from nanogram amounts of viral DNA, nearly 30 Mb of DNA sequence from two of these hot springs was determined. Between 400 and 1400 different viral types probably inhabit each hot spring.
More...Thermophilic viruses (phages) were first reported decades ago; however, our knowledge of their diversity, biology and ecological significance is still very limited. Previous research on thermophilic viruses has focused on cultured viruses. This study examined the genomic profiles and morphological diversity of uncultured viruses directly isolated from 74° to 93°C alkaline hot springs in Yellowstone National Park. Viral abundance ranged from 1E5 to 1E6 particles per milliliter, and a variety of bacteriophage and crenarchaeal virus-like morphologies were observed. Using a new method for constructing metagenomic libraries from nanogram amounts of viral DNA, nearly 30 Mb of DNA sequence from two of these hot springs was determined. Between 400 and 1400 different viral types probably inhabit each hot spring. Although most sequences were unrelated to known viral or cellular genomic sequences, hundreds of BLASTx similarities to genes associated with replication, recombination, lysis, transcription, lysogeny, and viral structural were identified. A significant portion of the viral metagenome was shared between the two hot springs, suggesting substantial overlap of the populations. Certain cultured viral genomes had remarkable similarity to the metagenomic sequences. Regions spanning almost the entire 28 kb genome of Pyrobacculum spherical virus have apparent homologs in libraries. Similarities to bacteriophages and acidophilic archaeal viruses isolated on other continents were limited to specific open reading frames, but were equally strong.
A collection of metagenomic sequences from viral communities from Bearpaw Hot Spring and Octopus Hot Spring in Yellowstone National Park. Less...
| Accession | PRJNA20821 |
| Type | Umbrella Metagenome project (Subtype:Metagenome) |
| Organism | hot springs metagenome[Taxonomy ID: 433727] unclassified sequences; metagenomes; ecological metagenomes; hot springs metagenome |
| Publications | Pride DT et al., "Genome signature analysis of thermal virus metagenomes reveals Archaea and thermophilic signatures.", BMC Genomics, 2008 Sep 17;9:420 |
| Submission | Registration date: 20-Sep-2007
JGI |
| Related Resources | |
Project Data:
| Resource Name | Number
of Links |
|---|
| Sequence data |
| SRA Experiments | 3 |
| Capillary Traces (Trace Archive) | 1 |
| Publications |
| PubMed | 1 |
| PMC | 1 |
| Other datasets |
| BioSample | 1 |
Viral Metagenome from Hot Springs encompasses the following 2 sub-projects:
| Project Type | Number of Projects |
| Metagenome | 2 |
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