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1 additional project is a component of the Dynamics of yeast ISWI and CHD chromatin remodeler genomic association.
Chromatin remodelers influence genetic processes by altering nucleosome occupancy, positioning, and composition. In vitro, yeast ISWI and CHD remodelers require > 20 bp of extranucleosomal DNA for remodeling, but linker DNA in S. cerevisiae averages < 20 bp. To resolve this paradox, we have mapped the genomic distributions of the yeast Isw1, Isw2, and Chd1 remodelers at base-pair resolution. Surprisingly, remodelers are highly enriched at promoter nucleosome depleted regions (5' NDRs), where they bind to regions of extended linker DNA. Remodelers are also enriched in the bodies of genes displaying high nucleosome turnover. We hypothesize that remodelers bind but do not act at 5' NDRs, remaining in physical proximity to gene bodies, where they act on regions of transient nucleosome depletion following transcriptional elongation.
Overall design: We have analyzed the dynamics of yeast ISWI and CHD chromatin remodeler genomic association at base-pair resolution using native chromatin immunoprecipitation followed by sequencing (N-ChIP-seq).
| Accession | PRJNA170934; GEO: GSE39430 |
| Data Type | Epigenomics |
| Scope | Multiisolate |
| Organism | Saccharomyces cerevisiae[Taxonomy ID: 4932] Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces; Saccharomyces cerevisiae |
| Publications | Zentner GE et al., "ISWI and CHD chromatin remodelers bind promoters but act in gene bodies.", PLoS Genet, 2013;9(2):e1003317 |
| Submission | Registration date: 17-Jul-2012
Henikoff, Basic Sciences, Fred Hutchinson Cancer Research Center |
| Relevance | Model Organism |
Project Data:
| Resource Name | Number
of Links |
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| Sequence data |
| SRA Experiments | 7 |
| Publications |
| PubMed | 1 |
| PMC | 1 |
| Other datasets |
| BioSample | 7 |
| GEO DataSets | 1 |