BioProject

Stress response is important to living organisms and a major aspect of natural selection in the wild. More...

Stress response is important to living organisms and a major aspect of natural selection in the wild. Identification of differentially expressed genes (DEG) and regulated pathways in respond to stressors is critical to understanding of fish biology. The fish intestine is one of the major organs that interacts with the environment and is involved in many biological processes. However, transcriptome in fish intestine has been poorly explored. In this study, we challenged Asian seabass, an important marine foodfish species, with LPS, Vibrio harveyi, high salinity and fasting, and sequenced six cDNA libraries of pooled intestine samples using Roche/454 GS FLX Titanium platform-based RNA-seq technology. A total of over 1 million high-quality sequence reads with an average reading length of 516 bp were obtained. We assembled and annotated the reads, and identified differentially expressed transcripts and regulated pathways under different stressors using a serial of bioinformatic tools. Less...