BioProject

The replication of eukaryotic chromosomes is organized temporally and spatially within the nucleus through epigenetic regulation of replication origin function. More...

The replication of eukaryotic chromosomes is organized temporally and spatially within the nucleus through epigenetic regulation of replication origin function. The characteristic initiation timing of specific origins is thought to reflect their chromatin environment or sub-nuclear positioning, however the mechanism remains obscure. Here we show that the yeast Forkhead transcription factors, Fkh1 and Fkh2, are global determinants of replication origin timing. Forkhead regulation of origin timing is independent of local levels or changes of transcription. Instead, we show that Fkh1 and Fkh2 are required for the clustering of early origins and their association with the key initiation factor Cdc45 in G1-phase, suggesting that Fkh1 and Fkh2 selectively recruit origins to emergent replication factories. Fkh1 and Fkh2 bind Fkh-activated origins, and interact physically with ORC, providing a plausible mechanism to cluster origins. These findings add a new dimension to our understanding of the epigenetic basis for differential origin regulation and its connection to chromosomal domain organization. Overall design: These files contain BrdU-IP-chip files from a BrdU-Pulse experiment (7 timepoints for each strain; 2 strains totall). These files also contain BrdU-IP-chip data from HU-arrested cells (1 replicate for 2 strains). These files also contain chIP-chip data of ORC (1 replicate 4 strains), MCM (1 replicate 2 strains), Cdc45 (1 replicate 2 stains), Fkh1 (1 replicate 2 strains). Finally, these files also contain 4C data (2 replicates 2 stains). Less...