BioProject

The genome sequencing strategy for the initial assembly (PRJNA12537) was a combined approach of Sanger whole genome shotgun (WGS) and BAC-end sequencing; the 5. More...

The genome sequencing strategy for the initial assembly (PRJNA12537) was a combined approach of Sanger whole genome shotgun (WGS) and BAC-end sequencing; the 5.2x coverage of the (clonable) macaque genome was derived from 14.9 Gb of sequence produced using Sanger sequencing methods. The Macaque Genome Sequencing Consortium was led by the Baylor College of Medicine Human Genome Sequencing Center in collaboration with the J. Craig Venter Joint Technology Center and the Genome Sequencing Center at Washington University in St. Louis. The WGS Sanger sequences have been deposited at GenBank/EMBL/DDBJ under the project accession AANU01000000.

The improved assembly developed by BCM Human Genome Sequencing Center started with prior rhesus genome data and assemblies and added Pacific Biosciences RS long reads (9x whole genome coverage) using DNA from the same reference animal. The RS reads were mapped to MacaM_Assembly_v7 (JSUE00000000) (Zimin, Cornish et al. 2014) and gaps filled using the PBJelly2 software (English, Richards et al. 2012). This has improved contig N50 substantially. Chromosome naming follows the conventional system of naming the rhesus macaque autosomes in order of decreasing size.