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ASM15366v1

Organism name:
Staphylococcus aureus subsp. aureus USA300_TCH959 (firmicutes)
Infraspecific name:
Strain: USA300_TCH959
BioSample:
SAMN00001491
Submitter:
Baylor College of Medicine
Date:
2009/08/12
Assembly level:
Scaffold
Genome representation:
full
GenBank assembly accession:
GCA_000153665.1 (latest)
RefSeq assembly accession:
GCF_000153665.1 (latest)
RefSeq assembly and GenBank assembly identical:
yes
WGS Project:
AASB02

IDs: 255168 [UID] 178298 [GenBank] 255168 [RefSeq]

See Genome Information for Staphylococcus aureus

There are 7442 assemblies for this organism

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History (Show revision history)

Comment

This sequence is generated as a part of the Human Microbiome Project (HMP), funded by the National Institutes of Health (NIH), National Human Genome Research Institute (NHGRI) Large-Scale Sequencing Research Network and the National Institute of Allergy and Infectious ... Diseases (NIAID) Microbial Genome Sequencing Centers Program. The mission of the HMP is to generate resources to enable the characterization of the human microbiota, the microbial communities living within human bodies, and analyze the role of these organisms in human health and disease. See http://www.hmpdacc.org/ for more information.
 The sequenced strain was obtained from Dr. Sheldon Kaplan (contact information: Texas Children's Hospital, Department of Pediatric Infectious Diseases, 1102 Bates Street #1150, Houston, TX, 77030; email: slkaplan@TexasChildrensHospital.org). The genomic DNA was prepared from a single bacterial isolate. The sequence generated included at least 10 fold coverage of Roche/454 Lifesciences FLX fragment data, at least 8 fold clone coverage of Roche/454 Lifesciences paired-end data and at least 10 fold coverage of Illumina/Solexa data.
 The Roche/454 Lifesciences sequence was assembled using the Roche/454 Lifescience Newbler assembler. The contigs from the Newbler assembly were aligned to the Illumina/Solexa data with mapping tools such as Mosaic and Crossmatch and these data were used for error correction for this version of the draft assembly. This draft assembly meets the HMP draft quality standards (more than 90% of the genome is included in contigs, more than 90% of a core set of bacterial genes are found with > 30% identity and > 30% length; more than 90% of the bases in the assembly have more than 5 fold sequence coverage, the contig N50 length is greater than 5kb, the scaffold N50 length is greater than 20kb, and there is less than 1 gap per 5kb).
 This is a reference genome for the Human Microbiome Project.
This project is co-owned with the Human Microbiome Project DACC.
Genome Coverage: 33x
Sequencing Technology: 454; ABI; Solexa/Illumina

Plasmid pUSA300HOUMS is GenBank Accession Number CP000732.
Annotation was added to the contigs in July 2009.  more

Global statistics

Total sequence length2,845,480
Total assembly gap length15,463
Gaps between scaffolds0
Number of scaffolds174
Scaffold N501,018,247
Scaffold L502
Number of contigs256
Contig N5054,777
Contig L5015
Total number of chromosomes and plasmids1

Supplemental Content

PubMed articles for this assembly

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Global assembly definition

Download the full sequence report
Assembly Unit: Primary Assembly (GCF_000153675.1)
Molecule nameGenBank sequenceRefSeq sequenceUnlocalized
sequences count
Plasmid pUSA300HOUMSCP000732.1=NC_010066.10
unplacedn/an/an/a173

Assembly statistics

MoleculeTotal
Length
Scaffold
Count
Ungapped
Length
Scaffold
N50
Spanned
Gaps
Unspanned
Gaps
Plasmid pUSA300HOUMS20,413120,41320,41300
unplaced2,825,0671732,809,6041,018,247820