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fSymMel2.1

Organism name:
Symphodus melops (corkwing wrasse)
BioSample:
SAMEA12219485
BioProject:
PRJEB58352
Submitter:
WELLCOME SANGER INSTITUTE
Date:
2022/12/21
Assembly type:
haploid (principal pseudohaplotype of diploid)
Assembly level:
Chromosome
Genome representation:
full
RefSeq category:
representative genome
GenBank assembly accession:
GCA_947650265.1 (latest)
RefSeq assembly accession:
n/a
RefSeq assembly and GenBank assembly identical:
n/a
WGS Project:
CANSXB01
Assembly method:
various
Genome coverage:
32x
Sequencing technology:
PacBio,Arima2
Linked assembly:
GCA_947650305.1 (alternate pseudohaplotype of diploid)

IDs: 15073651 [UID] 38905318 [GenBank]

See Genome Information for Symphodus melops

There are 4 assemblies for this organism

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History (Show revision history)

Comment

The assembly fSymMel2.1 is based on 32x PacBio data and Arima2 Hi-C data generated by the Darwin Tree of Life Project
(https://www.darwintreeoflife.org/). The assembly process included the following sequence of steps: initial PacBio assembly generation with Hifiasm, retained haplotig separation ... with purge_dups, and Hi-C based scaffolding with YaHS. The mitochondrial genome was assembled using MitoHiFi. Finally, the primary assembly was analysed and manually improved using rapid curation. Chromosome-scale scaffolds confirmed by the Hi-C data have been named in order of size. GOAT synteny (n=24) incorrect. Observed karyotype (n=23) matches cytometry results here:
https://www.researchgate.net/profile/Guillermo-Thode/publication/237187799_ Karyotype_divergence_in_Symphodus_melops_and_Symphodus_roissali_Labridae_Pe rciforms_C-banded_and_Ag-NOR_karyotypes/links/0c96051f2307708a8e000000/Kary otype-divergence-in-Symphodus-melops-and-Symphodus-roissali-Labridae-Percif orms-C-banded-and-Ag-NOR-karyotypes.pdf  more

Global statistics

Total sequence length636,368,453
Total ungapped length636,092,653
Gaps between scaffolds0
Number of scaffolds395
Scaffold N5027,641,414
Scaffold L5010
Number of contigs1,774
Contig N501,186,940
Contig L50156
Total number of chromosomes and plasmids24
Number of component sequences (WGS or clone)395

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Global assembly definition

Download the full sequence report
Click on the table row to see sequence details in the table to the right
Assembly Unit Name
Primary Assembly
non-nuclear
Assembly Unit: Primary Assembly (GCA_947650264.1)
Molecule nameGenBank sequenceRefSeq sequenceUnlocalized
sequences count
Chromosome 1OX393525.1n/an/a0
Chromosome 2OX393526.1n/an/a0
Chromosome 3OX393527.1n/an/a0
Chromosome 4OX393528.1n/an/a0
Chromosome 5OX393529.1n/an/a0
Chromosome 6OX393530.1n/an/a0
Chromosome 7OX393531.1n/an/a0
Chromosome 8OX393532.1n/an/a0
Chromosome 9OX393533.1n/an/a0
Chromosome 10OX393534.1n/an/a0
Chromosome 11OX393535.1n/an/a0
Chromosome 12OX393536.1n/an/a0
Chromosome 13OX393537.1n/an/a0
Chromosome 14OX393538.1n/an/a0
Chromosome 15OX393539.1n/an/a0
Chromosome 16OX393540.1n/an/a0
Chromosome 17OX393541.1n/an/a0
Chromosome 18OX393542.1n/an/a0
Chromosome 19OX393543.1n/an/a0
Chromosome 20OX393544.1n/an/a8
Chromosome 21OX393545.1n/an/a0
Chromosome 22OX393546.1n/an/a0
Chromosome 23OX393547.1n/an/a0
unplacedn/an/an/a363

Assembly statistics

MoleculeSequence RoleTotal
Length
Scaffold
Count
Ungapped
Length
Scaffold
N50
Spanned
Gaps
Unspanned
Gaps
AllAssembled molecule636,351,954394636,076,15427,641,4141,3790
Chromosome 1Assembled molecule56,958,096156,936,09656,958,0961100
Chromosome 2Assembled molecule31,721,703131,706,70331,721,703750
Chromosome 3Assembled molecule31,245,555131,235,15531,245,555520
Chromosome 4Assembled molecule30,971,358130,960,35830,971,358550
Chromosome 5Assembled molecule30,007,886129,995,08630,007,886640
Chromosome 6Assembled molecule29,433,924129,420,52429,433,924670
Chromosome 7Assembled molecule29,419,186129,410,38629,419,186440
Chromosome 8Assembled molecule29,035,277129,021,27729,035,277700
Chromosome 9Assembled molecule27,804,104127,792,90427,804,104560
Chromosome 10Assembled molecule27,641,414127,630,01427,641,414570
Chromosome 11Assembled molecule26,498,843126,489,04326,498,843490
Chromosome 12Assembled molecule26,391,557126,381,35726,391,557510
Chromosome 13Assembled molecule26,261,251126,250,45126,261,251540
Chromosome 14Assembled molecule25,701,058125,689,85825,701,058560
Chromosome 15Assembled molecule25,671,663125,659,66325,671,663600
Chromosome 16Assembled molecule25,028,912125,016,91225,028,912600
Chromosome 17Assembled molecule24,065,029124,053,82924,065,029560
Chromosome 18Assembled molecule23,128,467123,116,86723,128,467580
Chromosome 19Assembled molecule22,560,283122,547,68322,560,283630
Chromosome 20AllAssembled moleculeUnlocalized scaffolds21,352,81220,485,488867,32491821,340,61220,473,688866,92420,485,48820,485,488118,90061592000
Chromosome 21Assembled molecule20,976,262120,964,86220,976,262570
Chromosome 22Assembled molecule20,018,971120,005,17120,018,971690
Chromosome 23Assembled molecule14,860,057114,854,05714,860,057300
unplacedAssembled molecule9,598,2863639,597,28632,74850
MoleculeTotal
Length
Scaffold
Count
Ungapped
Length
Scaffold
N50
Spanned
Gaps
Unspanned
Gaps
Mitochondrion MT16,499116,49916,49900