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IDs: 11057641 [UID] 29214098 [GenBank]
The assembly ilMesFuru1.1 is based on 24x PacBio data, 10X Genomics Chromium data, and Arima Hi-C data generated by the Darwin Tree of Life Project (https://www.darwintreeoflife.org/). The assembly process included the following sequence of steps: initial PacBio assembly generation ... with Hifiasm, retained haplotig separation with purge_dups, short-read polishing using FreeBayes-called variants from 10X Genomics Chromium reads aligned with LongRanger, and Hi-C based scaffolding with SALSA2. The mitochondrial genome was assembled using MitoHifi. Finally, the assembly was analysed and manually improved using rapid curation. Chromosome-scale scaffolds confirmed by the Hi-C data have been named in order of size. Linkage has been observed in the Hi-C map between chromosomes 8 and 25. This linkage is specific, such that an alternative karyotype could be constructed {8A:8B,25A:25B} and {8B:25A,8A,25B}. No support for this fusion between chromosome 8 and 25 can be seen in the PacBio reads which derive from a different sample to the Hi-C. more
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