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IDs: 17612271 [UID] 45145628 [GenBank]
The assembly ucDunPrim2.2 is based on 40x PacBio data, 10X Genomics Chromium data, and Arima Hi-C data generated by the Darwin Tree of Life Project (https://www.darwintreeoflife.org/). The assembly process included the following sequence of steps: initial PacBio assembly generation ... with Hifiasm, retained haplotig separation with purge_dups, short-read polishing using FreeBayes-called variants from 10X Genomics Chromium reads aligned with LongRanger, and Hi-C based scaffolding with SALSA2. The mitochondrial and chloroplast genomes were assembled using MBG from PacBio HiFi reads mapping to related genomes. A representative circular sequence was selected for each from the graph based on read coverage. Finally, the primary assembly was analysed and manually improved using rapid curation. Chromosome-scale scaffolds confirmed by the Hi-C data have been named in order of size. more
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