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IDs: 12444881 [UID] 32586338 [GenBank]
The Oat_OT3098_v2 release is based on an improved contig assembly using the same PacBio HiFi sequence dataset, but with the hifiasm assembler. The Oat_OT3098_v2 assembly exhibits a significant improvement in contiguity (1343 contigs, contig N50 = 71 Mb, total ... size = 10.75 Gb). This assembly was scaffolded with the same BioNano maps as the unreleased Oat_OT3098_v1 assembly. However, the improved contigs significantly improved the contiguity of the scaffolds as well (84 scaffolds, scaffold N50= 374 kb, total size = 10.83 Gb). The Oat_OT3098_v2 assembly was manually curated to resolve overlapping contigs that were not addressed during BioNano hybrid scaffolding. Scaffolds were placed into pseudomolecules using the unreleased Oat_OT3098_v1 chromosomes as a guide and were validated by HiC contact map visualization. more
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