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IDs: 217061 [UID] 1361568 [GenBank] 1676668 [RefSeq]
Background: The golden eagle (Aquila chrysaetos) is one of the best-known birds of prey in the Northern Hemisphere. It is the most widely distributed species of eagle, occurring throughout Europe, Asia, northern Africa and North America. Golden eagles and ... their conservation are very important to Native Americans, because of the eagle's prominence in the culture of so many tribes, including the Iowa tribe of Oklahoma. The Iowa Tribe has a vested interest in the conservation of the golden eagle as their Grey Snow Eagle House's mission includes preserving the golden eagle through conservation. Therefore, the Iowa Tribe is funding the sequencing and assembly of the genome, which represents an initial step towards elucidating the relatively unknown golden eagle population structure. The Golden Eagle, Aquila chrysaetos canadensis, DNA blood sample was obtained from a male with a Oklahoma State Unversity ID of GSEH35GE. Total sequence assembled coverage of Illumina instrument reads was 88X (frags, 3kb and 8kb inserts) using a genome size estimate of 1.2Gb. The combined sequence reads were assembled using AllPaths_LG (Gnerre et al). Contamination was removed by screening the assembly against TGI (WUSTL) assemblies, several bacterial genomes and Illumina adapter sequences. Potential contaminants were confirmed by blasting against the NCBI nr/nt sequences. This draft assembly was referred to as Aquila chrysaetos 1.0.2. The assembly is made up of a total of 1141 scaffolds with an N50 scaffold length of almost 9.2Mb and an N50 contig length of over 172Kb. The assembled genome spans almost 1.2Gb when including gaps. DNA samples can be obtained from: Dr. Ronald A. Van Den Bussche Department of Zoology 501 Life Science West Oklahoma State University Stillwater, OK 74078 Email: ron.van_den_bussche@okstate.edu For questions regarding this Golden Eagle assembly please contact Dr. Wesley Warren, Washington University School of Medicine (wwarren@genome.wustl.edu). Downloads of the sequence data are available via the NCBI SRA database. Credits: DNA source - Dr. Ronald A. Van Den Bussche, Oklahoma State University Sequencing - The Genome Institute, Washington University School of Medicine, St Louis, MO. Sequence assembly - The Genome Institute, Washington University School of Medicine, St Louis, MO Citation upon use of this assembly in a manuscript: It is requested that users of this Aquila chrysaetos 1.0.2 sequence assembly acknowledge Dr. Wesley Warren and The Genome Institute, Washington University School of Medicine in any publications that result from use of this sequence assembly. Aquila chrysaetos 1.0.2 assembly stats: *** Contiguity: Contig *** Total contig number: 17031 Total contig bases: 1180019022 bp Average contig length: 69287 bp Maximum contig length: 1113908 bp N50 contig length: 172329 bp N50 contig number: 2004 *** Contiguity: Supercontig *** Total supercontig number: 1141 Average supercontig length: 1034197 bp Maximum supercontig length: 30467530 bp N50 supercontig length: 9157546 bp N50 supercontig number: 40 *** Scaffold Distribution*** Scaffolds > 1M: 174 Scaffold 250K--1M: 63 Scaffold 100K--250K: 33 Scaffold 10--100K: 158 Scaffold 5--10K: 100 Scaffold 2--5K: 242 Scaffold 0--2K: 371 more
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