Format

Send to:

Choose Destination

Download Assembly



Rhodnius_prolixus-3.0.3

Organism name:
Rhodnius prolixus (bugs)
BioSample:
SAMN02953759
BioProject:
PRJNA13648
Submitter:
Washington University Genome Sequencing Center
Date:
2015/04/08
Assembly level:
Scaffold
Genome representation:
full
RefSeq category:
representative genome
GenBank assembly accession:
GCA_000181055.3 (latest)
RefSeq assembly accession:
n/a
RefSeq assembly and GenBank assembly identical:
n/a
WGS Project:
ACPB03
Assembly method:
Cabog v. March 2015
Genome coverage:
8.3x
Sequencing technology:
454; Sanger dideoxy sequencing; Illumina

IDs: 321521 [UID] 1725298 [GenBank]

See Genome Information for Rhodnius prolixus

History (Show revision history)

Comment

Background:
 Rhodnius prolixus is an insect of the subfamily Triatominae, and an important vector of the Chagas parasite, Typanosoma cruzi. DNA was isolated by Ellen Dotson laboratory from animals from the colony at the Centers for Disease Control in ... Atlanta, GA. The whole-genome shotgun plasmid, BAC end reads and 454 reads were assembled using Cabog assembler generating an assembly (3.0.1) with a coverage of 8.1X. Illumina 700bp short insert were sequenced to gap fill using a custom script, and Illumina 8kbs were used to scaffold the assembly using SSPACE. The assembly was screened for contamination, and <200bp length reads were removed before submission. The final 3.0.3 assembly has a coverage of 8.3X, and is made up of a total of 16,537 scaffolds with an N50 scaffold length of 849,348bp (which includes singletons), and an N50 contig length of 35,751bp. The current improved 3.0.3 assembled genome spans nearly 707Mb of the expected 733Mb.
 For questions regarding this Rhodnius prolixus 3.0.3 assembly please contact Dr. Wesley Warren, Washington University School of Medicine (wwarren@genome.wustl.edu). Funding for the sequence characterization of the Rhodnius prolixus genome was provided by the National Human Genome Research Institute (NHGRI), National Institutes of Health (NIH).
 Source DNA can be obtained by mailing: Ellen Dotson Centers for Disease Control and Prevention Division of Parasitic Diseases CDC Chamblee Facility F22 4770 Buford Highway Atlanta, GA
 Credits:
 DNA source - Ellen Dotson, Centers for Disease Control and Prevention, Atlanta, GA
 Sequencing - The Genome Institute, Washington University School of Medicine, St Louis, MO. 
 Sequence assembly - The Genome Institute, Washington University School of Medicine, St Louis, MO.
 Citation upon use of this assembly in a manuscript: 
 Please refer to the Genome Institute Data Use Policy (http://genome.wustl.edu/data/data_use_policy) for further information. 

 Assembly Stats:
 *** Contiguity: Contig *** Total contig number: 47726 Total contig bases: 564634549 bp Average contig length: 11831 bp Maximum contig length: 373010 bp N50 contig length: 35751 bp N50 contig number: 4418
 *** Contiguity: Supercontig *** Total supercontig number: 16537 Average supercontig length: 34144 bp Maximum supercontig length: 11641147 bp N50 supercontig length: 849348 bp (includes single contig scaffolds or singletons) N50 supercontig number: 170
 Scaffolds > 1M: 137 Scaffold 250K--1M: 342 Scaffold 100K--250K: 210 Scaffold 10--100K: 2216 Scaffold 5--10K: 1074 Scaffold 2--5K: 2833 Scaffold 0--2K: 9725

  more

Global statistics

Total sequence length706,824,083
Total ungapped length564,634,549
Gaps between scaffolds0
Number of scaffolds16,537
Scaffold N501,088,772
Scaffold L50170
Number of contigs47,726
Contig N5035,751
Contig L504,418
Total number of chromosomes and plasmids0
Number of component sequences (WGS or clone)47,726

Supplemental Content

Recent activity

Your browsing activity is empty.

Activity recording is turned off.

Turn recording back on

See more...

Global assembly definition

Download the full sequence report
Click on the table row to see sequence details in the table to the right
Assembly Unit Name
Primary Assembly
The primary assembly unit does not have any assembled chromosomes or linkage groups.
Please download the full sequence report for information on the scaffolds.

Assembly statistics

MoleculeTotal
Length
Scaffold
Count
Ungapped
Length
Scaffold
N50
Spanned
Gaps
Unspanned
Gaps
unplaced706,824,08316,537564,634,5491,088,77231,1890
Support Center