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An organized view of the transcripome



Library:20953 (dbEST ID)

NICHD_XGC_tropTe1

Library Description

Organism:Xenopus (Silurana) tropicalis
Strain:TGA IC
Developmental stage:Adult
Tissue:Testes
Vector:pCS107
Cloning site 1:SalI
Cloning site 2:NotI
Lab host:ElectroMAX DH10B T1 Phage Resistant cells

This library was made from dT primed cDNA and cloned into pCS107 vector. The work was done at DOE Joint Genome Institute. Poly A RNA were primed with 5' GACTAGTTCTAGATCGCGAGCGGCCGCCCTTTTTTTTTTTTTTT 3'. CDNA were ligated to SalI adapter (5' TCGACCCACGCGTCCG and 5'CGGACGCGTGGG), digested with NotI, size fractionated in 1.1% agarose gel electrophoresis and ligated into NotI and SalI digested pCS107 vector.

Source: Lindquist,E.A., Richardson,P., DOE Joint Genome Institute

Gene Content Analysis

22,961 ESTs from this library were grouped into 6,618 UniGene entries (putative genes) [UniGene build #52, 23-Feb-2013]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs TPM   UniGene Entry
146 6359 Str.45491 Transcribed locus, strongly similar to NP_001017795.1 eef1a1b gene product [Danio rerio].
117 5096 Str.5646 Ribosomal protein, large, P0.
116 5052 Str.68954 Relaxin family locus C type II.
106 4617 Str.78278 Uncharacterized LOC100490730.
104 4529 Str.6701 Guanine nucleotide binding protein (G protein), beta polypeptide 2-like 1.
100 4355 Str.32162 Eukaryotic translation elongation factor 2, gene 1.
93 4050 Str.643 Ribosomal protein L3.
87 3789 Str.37180 Eukaryotic translation elongation factor 1 alpha 1.
83 3615 Str.65118 Ferritin, heavy polypeptide 1.
77 3354 Str.38707 CDNA clone IMAGE:8854248.
71 3092 Str.6666 Actin, gamma 1.
68 2962 Str.21593 H1 histone family, member X.
64 2787 Str.66547 Uncharacterized protein MGC146443.
62 2700 Str.20553 Ribosomal protein L5.
60 2613 Str.48519 Poly(A) binding protein, cytoplasmic 1.
60 2613 Str.26251 Cold inducible RNA binding protein.
57 2482 Str.68976 Actin,beta.
56 2439 Str.6703 Ribosomal protein SA.
54 2352 Str.64795 Tumor protein, translationally-controlled 1.
48 2091 Str.64764 Transcribed locus, strongly similar to NP_001079367.1 nuclease-sensitive element-binding protein 1 [Xenopus laevis].
46 2003 Str.64787 Ribosomal protein S2.
44 1916 Str.1803 Cyclin G1.
41 1786 Str.73758 Cytochrome P450, family 17, subfamily A, polypeptide 1.
41 1786 Str.70566 Heat shock protein 90kDa alpha (cytosolic), class A member 1, gene 1.
39 1699 Str.26758 SERPINE1 mRNA binding protein 1.
38 1655 Str.25131 Cofilin 1 (non-muscle).
37 1611 Str.6015 DEAD (Asp-Glu-Ala-Asp) box helicase 21.
36 1568 Str.681 Polr3e protein.
36 1568 Str.5144 High mobility group box 3.
35 1524 Str.47687 Y box binding protein 1.
35 1524 Str.4766 Adenine nucleotide translocase.
35 1524 Str.34175 Heat shock protein 90kDa alpha (cytosolic), class B member 1.
34 1481 Str.6165 Deleted in azoospermia-like.
33 1437 Str.47464 Eukaryotic translation elongation factor 1 gamma.
31 1350 Str.46699 Finished cDNA, clone TNeu077n09.
31 1350 Str.35931 Eukaryotic translation initiation factor 3, subunit D.
30 1307 Str.52532 Carbonic anhydrase I.
29 1263 Str.8811 Transcribed locus, strongly similar to NP_001080545.1 60S ribosomal protein L4-A [Xenopus laevis].
29 1263 Str.68962 Embryonic protein UVS.2-like.
29 1263 Str.47505 Eukaryotic translation initiation factor 5A.
28 1219 Str.8252 Ribosomal protein L7a.
28 1219 Str.46704 Chaperonin containing TCP1, subunit 2 (beta).
28 1219 Str.35958 Medium-chain specific acyl-CoA dehydrogenase, mitochondrial-like.
28 1219 Str.34567 DNA damage-regulated autophagy modulator protein 1-like.
28 1219 Str.10372 Calmodulin 2 (phosphorylase kinase, delta).
27 1176 Str.68009 Uncharacterized LOC100497586.
27 1176 Str.47265 Serine/arginine-rich splicing factor 5.
27 1176 Str.25041 Eukaryotic translation initiation factor 3, subunit E.
26 1132 Str.57495 Acid phosphatase 2, lysosomal.
26 1132 Str.51664 Uncharacterized LOC100135223.
26 1132 Str.45747 DEAD (Asp-Glu-Ala-Asp) box polypeptide 4.
26 1132 Str.417 Protein phosphatase 2, regulatory subunit A, alpha.
25 1089 Str.47018 RAN, member RAS oncogene family.
24 1045 Str.67707 Fas apoptotic inhibitory molecule 2.
24 1045 Str.6564 Ornithine decarboxylase antizyme 1.
24 1045 Str.26746 NME/NM23 family member 7.
23 1002 Str.6709 Eukaryotic translation initiation factor 3, subunit F.
23 1002 Str.6243 Protein tyrosine phosphatase type IVA, member 1.
23 1002 Str.48274 Tubulin, alpha 1c.
23 1002 Str.38523 Embryonic protein UVS.2-like.
22 958 Str.716 Synaptophysin-like 1.
22 958 Str.5930 Protein disulfide isomerase family A, member 3.
22 958 Str.5739 Ribosomal protein S3.
22 958 Str.51302 Enolase 1, (alpha).
22 958 Str.5048 Protein phosphatase 1, catalytic subunit, gamma isozyme.
21 915 Str.68404 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase.
21 915 Str.64812 Y box binding protein 2.
21 915 Str.55337 Tubulin, alpha 4a.
21 915 Str.44232 Glyceraldehyde-3-phosphate dehydrogenase.
21 915 Str.26145 Poly(rC) binding protein 3.
21 915 Str.11270 Tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon polypeptide.
21 915 Str.10351 Novel protein similar to prothymosin, alpha.
20 871 Str.65705 Cyclin-dependent kinase 1.
20 871 Str.64870 Fragile X mental retardation, autosomal homolog 1.
20 871 Str.6150 Voltage-dependent anion channel 2.
20 871 Str.39900 High mobility group box 2.
20 871 Str.38721 Uncharacterized LOC100486380.
20 871 Str.16917 Eukaryotic translation initiation factor 1.
19 827 Str.6682 Fibronectin 1.
19 827 Str.6522 Protein phosphatase 2, catalytic subunit, beta isozyme.
19 827 Str.6096 Ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase).
19 827 Str.59414 Uncharacterized LOC100127658.
19 827 Str.45809 Heterogeneous nuclear ribonucleoprotein A/B.
19 827 Str.4536 Serine/threonine-protein kinase 35-like.
19 827 Str.16646 Shwachman-Bodian-Diamond syndrome.
18 784 Str.62811 Transcribed locus.
18 784 Str.60300 Uncharacterized protein MGC75752.
18 784 Str.5710 Eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa.
18 784 Str.52469 Ribosomal protein S3A.
18 784 Str.46889 DEAD (Asp-Glu-Ala-Asp) box polypeptide 3, X-linked.
18 784 Str.373 Pyruvate kinase, muscle.
18 784 Str.21640 Cell cycle progression 1.
17 740 Str.7403 Chaperonin subunit 3 (gamma).
17 740 Str.68912 Retinoic acid receptor responder (tazarotene induced) 1.
17 740 Str.6679 GTPase activating protein (SH3 domain) binding protein 2.
17 740 Str.65384 Transcribed locus, strongly similar to NP_033739.1 actin, cytoplasmic 2 [Mus musculus].
17 740 Str.65146 Ribosomal protein S4, X-linked.
17 740 Str.6170 Secreted protein, acidic, cysteine-rich (osteonectin).
17 740 Str.527 Glioma tumor suppressor candidate region gene 2.
17 740 Str.51878 Uncharacterized LOC733778.

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Source: NCBI UniGene

EST Sequences

23,796 EST sequences from this library have been submitted to the public sequence database as of 23-Feb-2013.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

  • testis
  • adult
  • non-normalized

Source: NCBI Clone Registry



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