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Library:24182 (dbEST ID)

Organism: | Peromyscus maniculatus bairdii |

Strain: | BW |

Developmental stage: | newborn |

Sex: | pooled male and female |

Tissue: | 3 pooled whole animals |

Vector: | pDNR-LIB |

Cloning site 1: | SfiIA |

Cloning site 2: | SfiIB |

Lab host: | DH10B T1-resistant |

The library was made from oligo(dT)-primed cDNA and cloned into vector pDNR-LIB (Clontech Laboratories, Inc., Mountain View, CA) using the SMART system (BD Biosciences, San Jose, CA). To generate the first strand, polyA RNA was primed with an oligo(dT) primer (5'-ATTCTAGAGGCCGAGGCGGCCGACATG(T)30 VN-3', where V = A, G, or C). The second strand was synthesized by PCR using primer 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' followed by digestion of the SfiI sites. cDNA was size selected using 1.1% agarose gel electrophoresis, then ligated into pDNR-LIB. Library is normalized. Library was created and sequencing performed by the DOE Joint Genome Institute (Walnut Creek, CA).

Source: Lindquist,E.A., DOE Joint Genome Institute

14,228 ESTs from this library were grouped into 3,893 UniGene entries (putative genes) [UniGene build #2, 09-Mar-2010]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs | TPM | UniGene Entry | ||
---|---|---|---|---|

106 | 7450 | Pmn.16339 | Transcribed locus, moderately similar to XP_003084764.1 PREDICTED: hemoglobin subunit beta-1-like isoform 4 [Mus musculus]. | |

32 | 2249 | Pmn.4354 | Transcribed locus, strongly similar to NP_001077424.1 hemoglobin alpha, adult chain 2 [Mus musculus]. | |

26 | 1827 | Pmn.7068 | Transcribed locus, strongly similar to NP_033138.1 protein S100-A10 [Mus musculus]. | |

26 | 1827 | Pmn.3268 | Transcribed locus, strongly similar to XP_001076104.2 PREDICTED: microsomal glutathione S-transferase 3 [Rattus norvegicus]. | |

24 | 1687 | Pmn.9959 | Transcribed locus, moderately similar to NP_001120641.1 transcription elongation factor A protein-like 7 [Mus musculus]. | |

24 | 1687 | Pmn.7069 | Transcribed locus, moderately similar to NP_064420.2 calmodulin-4 [Mus musculus]. | |

24 | 1687 | Pmn.6141 | Transcribed locus, strongly similar to NP_001003405.1 trypsin 5 [Mus musculus]. | |

24 | 1687 | Pmn.3583 | Transcribed locus, strongly similar to NP_001007968.1 60S ribosomal protein L30 [Gallus gallus]. | |

24 | 1687 | Pmn.3079 | Transcribed locus, strongly similar to XP_423225.1 PREDICTED: similar to rat ribosomal protein L9 homologue [Gallus gallus]. | |

24 | 1687 | Pmn.3006 | Transcribed locus, strongly similar to NP_001014297.1 transcription elongation factor A protein-like 8 [Rattus norvegicus]. | |

24 | 1687 | Pmn.2228 | Transcribed locus, strongly similar to NP_058898.1 cytochrome c oxidase subunit 4 isoform 1, mitochondrial precursor [Rattus norvegicus]. | |

23 | 1617 | Pmn.555 | Transcribed locus, strongly similar to NP_058849.1 40S ribosomal protein S3a [Rattus norvegicus]. | |

23 | 1617 | Pmn.3894 | Transcribed locus, weakly similar to NP_073199.1 prolactin-inducible protein homolog precursor [Rattus norvegicus]. | |

23 | 1617 | Pmn.2738 | Transcribed locus, strongly similar to NP_990284.1 ubiquitin-40S ribosomal protein S27a [Gallus gallus]. | |

22 | 1546 | Pmn.6855 | Transcribed locus, strongly similar to NP_001026156.1 heterogeneous nuclear ribonucleoproteins A2/B1 [Gallus gallus]. | |

22 | 1546 | Pmn.3636 | Transcribed locus, strongly similar to NP_001093115.1 60S ribosomal protein L22-like 1 [Homo sapiens]. | |

22 | 1546 | Pmn.16315 | Transcribed locus, strongly similar to NP_064335.1 signal peptidase complex catalytic subunit SEC11A [Mus musculus]. | |

21 | 1476 | Pmn.8100 | Transcribed locus, moderately similar to NP_032522.2 galectin-7 [Mus musculus]. | |

21 | 1476 | Pmn.7404 | Transcribed locus, moderately similar to NP_001078821.1 apolipoprotein C-II [Rattus norvegicus]. | |

21 | 1476 | Pmn.16296 | Transcribed locus, strongly similar to NP_001009480.1 cytochrome b-c1 complex subunit 6, mitochondrial [Rattus norvegicus]. | |

21 | 1476 | Pmn.126 | Transcribed locus, strongly similar to NP_034490.1 glutathione S-transferase Mu 5 [Mus musculus]. | |

21 | 1476 | Pmn.1192 | Transcribed locus, strongly similar to NP_035421.2 60S ribosomal protein L7 [Mus musculus]. | |

20 | 1406 | Pmn.785 | Transcribed locus, strongly similar to NP_034075.2 cytochrome c oxidase subunit 7A2, mitochondrial precursor [Mus musculus]. | |

20 | 1406 | Pmn.5328 | Transcribed locus, strongly similar to NP_057016.1 proteasome maturation protein [Homo sapiens]. | |

20 | 1406 | Pmn.3351 | Transcribed locus, moderately similar to XP_001074466.1 PREDICTED: similar to ATP synthase, H+ transporting, mitochondrial F0 complex, subunit f, isoform 2 [Rattus norvegicus]. | |

20 | 1406 | Pmn.2311 | Transcribed locus, strongly similar to NP_001032423.1 40S ribosomal protein S19 [Rattus norvegicus]. | |

20 | 1406 | Pmn.2060 | Transcribed locus, strongly similar to NP_058974.1 proteasome subunit alpha type-1 [Rattus norvegicus]. | |

19 | 1335 | Pmn.774 | Transcribed locus, strongly similar to NP_031615.1 calmodulin [Mus musculus]. | |

19 | 1335 | Pmn.7354 | Transcribed locus, moderately similar to XP_001478505.1 PREDICTED: ATP synthase subunit g, mitochondrial-like [Mus musculus]. | |

19 | 1335 | Pmn.6934 | Transcribed locus, moderately similar to NP_803421.1 3-ketoacyl-CoA thiolase, mitochondrial [Mus musculus]. | |

19 | 1335 | Pmn.3889 | Transcribed locus, strongly similar to NP_032186.2 glutathione peroxidase 1 [Mus musculus]. | |

19 | 1335 | Pmn.3557 | Transcribed locus, strongly similar to NP_033118.1 40S ribosomal protein S17 [Mus musculus]. | |

19 | 1335 | Pmn.2690 | Transcribed locus, moderately similar to NP_081234.2 SRA stem-loop-interacting RNA-binding protein, mitochondrial precursor [Mus musculus]. | |

19 | 1335 | Pmn.2450 | Transcribed locus, strongly similar to NP_003964.3 60S ribosomal protein L14 [Homo sapiens]. | |

18 | 1265 | Pmn.6580 | Transcribed locus, strongly similar to NP_001001969.1 lymphocyte antigen 6 complex G6C [Rattus norvegicus]. | |

18 | 1265 | Pmn.6427 | Transcribed locus, strongly similar to XP_002664732.1 PREDICTED: ribosomal protein S5-like [Danio rerio]. | |

18 | 1265 | Pmn.3321 | Transcribed locus, strongly similar to XP_416113.1 PREDICTED: similar to Rps16 protein [Gallus gallus]. | |

18 | 1265 | Pmn.2462 | Transcribed locus, strongly similar to XP_002726835.1 PREDICTED: ribosomal protein L35a-like [Rattus norvegicus]. | |

18 | 1265 | Pmn.2316 | Transcribed locus, strongly similar to NP_001002079.1 40S ribosomal protein S13 [Danio rerio]. | |

18 | 1265 | Pmn.2133 | Transcribed locus, strongly similar to NP_035424.1 activated RNA polymerase II transcriptional coactivator p15 [Mus musculus]. | |

18 | 1265 | Pmn.2066 | Transcribed locus, strongly similar to NP_001002097.1 probable protein BRICK1 [Danio rerio]. | |

17 | 1195 | Pmn.8545 | Transcribed locus, moderately similar to NP_001012359.1 angiogenin, ribonuclease, RNase A family, 5 [Rattus norvegicus]. | |

17 | 1195 | Pmn.6691 | Transcribed locus, strongly similar to NP_059068.1 lysozyme C-2 precursor [Mus musculus]. | |

17 | 1195 | Pmn.5258 | Transcribed locus, strongly similar to NP_958818.1 60S ribosomal protein L17 [Rattus norvegicus]. | |

17 | 1195 | Pmn.4933 | Transcribed locus, moderately similar to XP_003364122.1 PREDICTED: 6.8 kDa mitochondrial proteolipid-like [Equus caballus]. | |

17 | 1195 | Pmn.423 | Transcribed locus, strongly similar to NP_058977.1 proteasome subunit alpha type-4 [Rattus norvegicus]. | |

17 | 1195 | Pmn.2811 | Transcribed locus, strongly similar to XP_002725396.1 PREDICTED: cytochrome c, somatic-like [Rattus norvegicus]. | |

17 | 1195 | Pmn.2546 | Transcribed locus, strongly similar to NP_038725.1 transthyretin [Mus musculus]. | |

17 | 1195 | Pmn.2380 | Transcribed locus, strongly similar to NP_056591.1 microfibrillar-associated protein 5 precursor [Mus musculus]. | |

17 | 1195 | Pmn.2163 | Transcribed locus, strongly similar to NP_061341.1 60S acidic ribosomal protein P1 [Mus musculus]. | |

17 | 1195 | Pmn.1116 | Transcribed locus, strongly similar to NP_033182.1 selenoprotein W [Mus musculus]. | |

16 | 1125 | Pmn.9860 | Transcribed locus. | |

16 | 1125 | Pmn.8991 | Transcribed locus, moderately similar to NP_038741.1 keratin-associated protein 15-1 [Mus musculus]. | |

16 | 1125 | Pmn.7785 | Transcribed locus, strongly similar to NP_036099.1 proteasome subunit alpha type-7 [Mus musculus]. | |

16 | 1125 | Pmn.7133 | Transcribed locus, strongly similar to NP_035826.1 voltage-dependent anion-selective channel protein 3 isoform 2 [Mus musculus]. | |

16 | 1125 | Pmn.5381 | Transcribed locus, strongly similar to NP_476443.1 ADP/ATP translocase 2 [Rattus norvegicus]. | |

16 | 1125 | Pmn.5117 | Transcribed locus, strongly similar to NP_031856.1 acyl-CoA-binding protein isoform 2 [Mus musculus]. | |

16 | 1125 | Pmn.4729 | Transcribed locus, strongly similar to NP_079655.1 cytochrome c oxidase subunit 7B, mitochondrial precursor [Mus musculus]. | |

16 | 1125 | Pmn.4094 | Transcribed locus, strongly similar to NP_001013079.1 adenine phosphoribosyltransferase [Rattus norvegicus]. | |

16 | 1125 | Pmn.2725 | Transcribed locus, strongly similar to NP_062798.1 actin-related protein 2/3 complex subunit 3 [Mus musculus]. | |

16 | 1125 | Pmn.2542 | Transcribed locus, strongly similar to NP_058975.1 proteasome subunit alpha type-2 [Rattus norvegicus]. | |

16 | 1125 | Pmn.2333 | Transcribed locus, strongly similar to NP_000981.1 60S ribosomal protein L27a [Homo sapiens]. | |

16 | 1125 | Pmn.205 | Transcribed locus, strongly similar to NP_058030.1 histone H2A.Z [Mus musculus]. | |

16 | 1125 | Pmn.16616 | Transcribed locus, moderately similar to NP_036644.1 beta-2-microglobulin precursor [Rattus norvegicus]. | |

16 | 1125 | Pmn.16385 | Transcribed locus, strongly similar to NP_000977.1 60S ribosomal protein L24 [Homo sapiens]. | |

16 | 1125 | Pmn.1513 | Transcribed locus, moderately similar to XP_002725066.1 PREDICTED: rCG31129-like [Rattus norvegicus]. | |

16 | 1125 | Pmn.1138 | Transcribed locus, strongly similar to NP_001016.1 40S ribosomal protein S23 [Homo sapiens]. | |

16 | 1125 | Pmn.10008 | Transcribed locus, strongly similar to NP_032616.1 mesoderm-specific transcript protein [Mus musculus]. | |

15 | 1054 | Pmn.9166 | Transcribed locus. | |

15 | 1054 | Pmn.6900 | Transcribed locus, strongly similar to NP_037036.1 annexin A1 [Rattus norvegicus]. | |

15 | 1054 | Pmn.5641 | Transcribed locus, strongly similar to NP_062723.1 gamma-aminobutyric acid receptor-associated protein [Mus musculus]. | |

15 | 1054 | Pmn.4573 | Transcribed locus, strongly similar to XP_344047.2 PREDICTED: similar to Ubiquitin-conjugating enzyme E2 L3 (Ubiquitin-protein ligase L3) (Ubiquitin carrier protein L3) (UbcM4) [Rattus norvegicus]. | |

15 | 1054 | Pmn.4331 | Transcribed locus, strongly similar to XP_002726748.1 PREDICTED: mitochondrial ribosomal protein L33-like [Rattus norvegicus]. | |

15 | 1054 | Pmn.3720 | Transcribed locus, strongly similar to NP_033120.1 40S ribosomal protein S4, X isoform [Mus musculus]. | |

15 | 1054 | Pmn.3709 | Transcribed locus, moderately similar to NP_001005534.1 succinate dehydrogenase complex, subunit C [Rattus norvegicus]. | |

15 | 1054 | Pmn.3604 | Transcribed locus, strongly similar to NP_035016.1 NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 4 [Mus musculus]. | |

15 | 1054 | Pmn.3518 | Transcribed locus, moderately similar to NP_037288.1 cathepsin L1 preproprotein [Rattus norvegicus]. | |

15 | 1054 | Pmn.2569 | Transcribed locus, strongly similar to NP_001005528.1 40S ribosomal protein S25 [Rattus norvegicus]. | |

15 | 1054 | Pmn.1735 | Transcribed locus, strongly similar to NP_001185781.1 forkhead box protein J3 isoform 2 [Homo sapiens]. | |

15 | 1054 | Pmn.16720 | Transcribed locus, weakly similar to NP_001020745.1 hypothetical protein LOC433492 [Mus musculus]. | |

15 | 1054 | Pmn.1663 | Transcribed locus, strongly similar to NP_001395.1 elongation factor 1-gamma [Homo sapiens]. | |

15 | 1054 | Pmn.16466 | Transcribed locus, strongly similar to NP_001988.1 ubiquitin-like protein fubi and ribosomal protein S30 precursor [Homo sapiens]. | |

15 | 1054 | Pmn.10096 | Transcribed locus, strongly similar to NP_001029296.1 microfibril-associated glycoprotein 4 [Rattus norvegicus]. | |

14 | 984 | Pmn.7638 | Transcribed locus, strongly similar to NP_115877.2 cornifelin [Homo sapiens]. | |

14 | 984 | Pmn.7381 | Transcribed locus, strongly similar to NP_110459.1 fatty acid-binding protein, brain [Rattus norvegicus]. | |

14 | 984 | Pmn.7096 | Transcribed locus, moderately similar to NP_058709.2 glutathione S-transferase alpha-1 [Rattus norvegicus]. | |

14 | 984 | Pmn.611 | Transcribed locus, strongly similar to NP_998369.1 40S ribosomal protein S20 [Danio rerio]. | |

14 | 984 | Pmn.5614 | Transcribed locus, strongly similar to NP_001003447.1 60S ribosomal protein L15 [Danio rerio]. | |

14 | 984 | Pmn.4087 | Transcribed locus, strongly similar to NP_001124478.1 signal peptidase complex subunit 1 [Rattus norvegicus]. | |

14 | 984 | Pmn.4074 | Transcribed locus, strongly similar to XP_001069423.2 PREDICTED: Ngg1 interacting factor 3 like 1 binding protein 1-like [Rattus norvegicus]. | |

14 | 984 | Pmn.3637 | Transcribed locus, strongly similar to NP_001017582.1 small nuclear ribonucleoprotein Sm D2 [Danio rerio]. | |

14 | 984 | Pmn.3225 | Transcribed locus, strongly similar to NP_005457.2 mediator of RNA polymerase II transcription subunit 6 [Homo sapiens]. | |

14 | 984 | Pmn.3167 | Transcribed locus, strongly similar to NP_001091274.1 interferon induced transmembrane protein 3 [Xenopus laevis]. | |

14 | 984 | Pmn.2939 | Transcribed locus, strongly similar to NP_778166.2 39S ribosomal protein L22, mitochondrial precursor [Mus musculus]. | |

14 | 984 | Pmn.2299 | Transcribed locus, strongly similar to NP_057182.1 brain protein 44-like protein [Homo sapiens]. | |

14 | 984 | Pmn.2291 | Transcribed locus, strongly similar to NP_000984.1 60S ribosomal protein L31 isoform 1 [Homo sapiens]. | |

14 | 984 | Pmn.16719 | Transcribed locus, strongly similar to NP_998515.1 ras homolog gene family, member Ac [Danio rerio]. | |

14 | 984 | Pmn.10881 | Transcribed locus, strongly similar to NP_001005550.1 NADH-ubiquinone oxidoreductase 75 kDa subunit, mitochondrial precursor [Rattus norvegicus]. | |

13 | 914 | Pmn.8523 | Transcribed locus, strongly similar to NP_082280.2 titin isoform N2-B [Mus musculus]. | |

13 | 914 | Pmn.8251 | Transcribed locus, moderately similar to NP_037033.1 protein AMBP precursor [Rattus norvegicus]. |

Source: NCBI UniGene

15,987 EST sequences from this library have been submitted to the public sequence database as of 09-Mar-2010.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)