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An organized view of the transcripome



Library:11266 (dbEST ID)

NIH_MGC_143

Library Description

Organism:Mus musculus
Organ:Brain
Vector:pDNR-LIB
Vector type:plasmid (chloramphenicol resistant, grown at 30C)
Cloning site 1:SfiI (ggccattatggcc)
Cloning site 2:SfiI (ggccgcctcggcc)
Lab host:DH10B (T1-phage-resistant)

cDNA made by oligo-dT priming and directionally cloned. 5' and 3' adaptors were used in cloning as follows: 5'-AAGCAGTGGTATCAACGCAGAGTGGCCATTACGGCCGGG-3' and 5'-ATTCTAGAGGCCGAGGCGGCCGACATG-dT(30)NN-3'. Full-length enriched library was constructed using the Clontech Creator SMART kit and size-selected to contain the 0.2-0.5 kb size fraction (other fractions present in NIH_MGC_144). Library created in the laboratory of M. Brownstein (NIMH, NIH). Note: this is a NIH_MGC Library.

Source: Robert Strausberg, Ph.D.

Gene Content Analysis

6,474 ESTs from this library were grouped into 2,624 UniGene entries (putative genes) [UniGene build #194, 25-Jan-2013]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs TPM   UniGene Entry
131 20235 Mm.441437 CDNA clone IMAGE:40049146.
56 8650 Mm.548 Cytochrome c oxidase subunit VIc.
52 8032 Mm.17851 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 3.
52 8032 Mm.136093 ATP synthase, H+ transporting, mitochondrial F1F0 complex, subunit e.
45 6951 Mm.455357 CDNA clone IMAGE:3587527.
42 6487 Mm.379119 Ubiquinol-cytochrome c reductase, complex III subunit XI.
41 6333 Mm.378898 Cytochrome c oxidase subunit VIIc.
40 6179 Mm.34869 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 1.
40 6179 Mm.29867 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 2.
38 5870 Mm.415865 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 4.
38 5870 Mm.133551 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F2.
37 5715 Mm.251621 Ubiquinol-cytochrome c reductase, complex III subunit VII.
36 5561 Mm.371592 Ubiquitin B.
36 5561 Mm.3532 Thymosin, beta 10.
36 5561 Mm.29722 Upregulated during skeletal muscle growth 5.
36 5561 Mm.21162 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13.
35 5406 Mm.14022 Cytochrome c oxidase subunit VIIIa.
33 5097 Mm.391967 Actin, beta.
32 4943 Mm.400 Cytochrome c oxidase, subunit VIb polypeptide 1.
31 4788 Mm.353 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit F.
31 4788 Mm.331007 NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 1.
30 4634 Mm.152627 Cytochrome c oxidase subunit VIIa 2.
29 4479 Mm.275780 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 5.
29 4479 Mm.197728 Cytochrome c oxidase subunit VIIb.
28 4325 Mm.5246 Peptidylprolyl isomerase A.
28 4325 Mm.2171 ATPase inhibitory factor 1.
27 4171 Mm.29415 NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2.
27 4171 Mm.153758 Transcription elongation factor B (SIII), polypeptide 2.
26 4016 Mm.345333 Ribosomal protein L35.
25 3862 Mm.455354 Transcribed locus, strongly similar to NP_036788.1 Sparc gene product [Rattus norvegicus].
25 3862 Mm.329243 Calmodulin 2.
25 3862 Mm.275831 Aldolase A, fructose-bisphosphate.
24 3707 Mm.269736 Ubiquinol-cytochrome c reductase, complex III subunit X.
24 3707 Mm.14663 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit g.
23 3553 Mm.290774 Heat shock protein 8.
23 3553 Mm.171123 Guanine nucleotide binding protein (G protein), gamma 13.
22 3398 Mm.405359 Tubulin, alpha 1A.
22 3398 Mm.379006 Ribosomal protein S29.
22 3398 Mm.29513 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 7 (B14.5a).
22 3398 Mm.290786 Ribosomal protein L41.
22 3398 Mm.2064 Metallothionein 3.
21 3244 Mm.458386 Transcribed locus, strongly similar to NP_001101855.1 dematin [Rattus norvegicus].
21 3244 Mm.335368 Transcribed locus, strongly similar to XP_003086687.2 PREDICTED: peptidyl-prolyl cis-trans isomerase A-like [Mus musculus].
21 3244 Mm.16831 Creatine kinase, brain.
20 3089 Mm.20841 ATP synthase, H+ transporting, mitochondrial F1 complex, epsilon subunit.
19 2935 Mm.392113 Tubulin, alpha 1B.
19 2935 Mm.289669 Ribosomal protein S21.
19 2935 Mm.270283 Ribosomal protein S27.
19 2935 Mm.192991 Metallothionein 1.
18 2780 Mm.491081 Transcribed locus, strongly similar to NP_904337.1 NADH dehydrogenase subunit 4 [Mus musculus].
18 2780 Mm.42805 NADH dehydrogenase (ubiquinone) Fe-S protein 5.
18 2780 Mm.285993 Calmodulin 1.
18 2780 Mm.28349 NADH dehydrogenase (ubiquinone) flavoprotein 3.
18 2780 Mm.279823 NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 11.
17 2626 Mm.379136 Ubiquinol-cytochrome c reductase binding protein.
17 2626 Mm.318 RIKEN cDNA 2010107E04 gene.
17 2626 Mm.291868 Translocase of outer mitochondrial membrane 7 homolog (yeast).
16 2471 Mm.455364 RNA binding site for Dazl protein, clone ba2.
16 2471 Mm.380115 Polymerase (RNA) II (DNA directed) polypeptide L.
16 2471 Mm.2180 Heat shock protein 90 alpha (cytosolic), class B member 1.
15 2317 Mm.390165 Ubiquitin-like 5.
15 2317 Mm.2785 Diazepam binding inhibitor.
15 2317 Mm.238817 Ribosomal protein L38.
14 2162 Mm.431935 Transcribed locus, strongly similar to NP_033119.1 Rps29 gene product [Mus musculus].
14 2162 Mm.42767 Ribosomal protein S17.
14 2162 Mm.390468 Predicted gene 2701.
14 2162 Mm.361657 Predicted gene 13826.
14 2162 Mm.276856 Ribosomal protein L11.
14 2162 Mm.27396 Cytochrome c oxidase assembly protein 17.
14 2162 Mm.272389 Mitochondrial nucleoid factor 1.
14 2162 Mm.140568 Ribosomal protein L34.
13 2008 Mm.5083 RAB3A, member RAS oncogene family.
13 2008 Mm.440882 Purkinje cell protein 2 (L7).
13 2008 Mm.386758 Cytochrome c oxidase subunit IV isoform 1.
13 2008 Mm.262252 Small EDRK-rich factor 2.
12 1854 Mm.45953 Synaptosomal-associated protein 25.
12 1854 Mm.419997 Ubiquitin C.
12 1854 Mm.389435 Ribosomal protein S11.
12 1854 Mm.384744 Transcribed locus, strongly similar to NP_904329.1 NADH dehydrogenase subunit 2 [Mus musculus].
12 1854 Mm.355701 SRA stem-loop interacting RNA binding protein.
12 1854 Mm.298 Solute carrier family 25 (mitochondrial carrier, phosphate carrier), member 3.
12 1854 Mm.24108 Mitochondrial ribosomal protein S21.
12 1854 Mm.181721 Ubiquinol-cytochrome c reductase hinge protein.
11 1699 Mm.380435 Ribosomal protein, large P2.
11 1699 Mm.379154 NADH dehydrogenase (ubiquinone) 1 beta subcomplex 4.
11 1699 Mm.371623 Reactive oxygen species modulator 1.
11 1699 Mm.337074 Myosin, light polypeptide 6, alkali, smooth muscle and non-muscle.
11 1699 Mm.325350 SEC61, gamma subunit.
11 1699 Mm.2395 Translational machinery associated 7 homolog (S. cerevisiae).
11 1699 Mm.138471 Eukaryotic translation elongation factor 1 alpha 1.
10 1545 Mm.9745 Lactate dehydrogenase B.
10 1545 Mm.491158 Transcribed locus, strongly similar to NP_904335.1 NADH dehydrogenase subunit 3 [Mus musculus].
10 1545 Mm.486178 Transcribed locus.
10 1545 Mm.3158 Ribosomal protein, large, P1.
10 1545 Mm.29897 NADH dehydrogenase (ubiquinone) Fe-S protein 6.
10 1545 Mm.297096 Malate dehydrogenase 2, NAD (mitochondrial).
10 1545 Mm.2966 ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C3 (subunit 9).
10 1545 Mm.288630 Calmodulin 3.
10 1545 Mm.282093 Ubiquitin pseudogene.
10 1545 Mm.274995 Small nuclear ribonucleoprotein N.

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Source: NCBI UniGene

EST Sequences

9,831 EST sequences from this library have been submitted to the public sequence database as of 25-Jan-2013.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

  • brain
  • unknown developmental stage

Source: NCBI Clone Registry



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