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An organized view of the transcripome



Library:3745 (dbEST ID)

Gm-r1030

Library Description

Organism:Glycine max
Vector:pSPORT1
Vector type:plasmid
Cloning site 1:SalI
Cloning site 2:NotI
Lab host:DH10B

This cDNA library was constructed from mRNA isolated from immature cotyledons of greenhouse grown plants (individual seed fresh weight of 100-300mg). The library was prepared using the Life Technologies pSuperScript cDNA library construction kit. Complementary DNA was synthesized from mRNA using a poly(dT) sequence with a NotI restriction site. SalI linkers adapters were ligated to the blunt-ended cDNA fragments followed by NotI digestion. The cDNA fragments were directionally cloned into the NotI-SalI restriction site of the pSPORT1 vector. The ligated cDNA fragments were transformed into E. coli ElectroMax DH10B host cells. This library was constructed by Dr. Lila Vodkin and Dr. Anu Khanna. Note that Gm-r1030 is a re-rack of Gm-c1007.

Source: Shoemaker R/Public Soybean EST Project, Washington University School of Medicine

Gene Content Analysis

4,112 ESTs from this library were grouped into 2,215 UniGene entries (putative genes) [UniGene build #42, 26-Feb-2012]. EST counts for each entry may be used to calculate an approximate expression level in transcripts per million (TPM).

ESTs TPM   UniGene Entry
90 21887 Gma.27137 Beta-conglycinin alpha prime subunit.
49 11916 Gma.595 Beta-amylase.
38 9241 Gma.30735 Uncharacterized LOC100305855.
38 9241 Gma.244 Alcohol dehydrogenase 1-like.
33 8025 Gma.26853 Beta-conglycinin beta-subunit.
30 7296 Gma.28867 Bowman-Birk proteinase isoinhibitor D-II.
29 7053 Gma.1889 16.5 kDa oleosin.
29 7053 Gma.11028 Formate dehydrogenase, mitochondrial-like.
28 6809 Gma.30739 Lipoxygenase-3.
28 6809 Gma.17525 Translationally controlled tumor-like protein.
25 6080 Gma.434 Cysteine proteinase.
25 6080 Gma.1839 Omega-6 fatty acid desaturase, endoplasmic reticulum isozyme 1-like.
25 6080 Gma.10984 Protein disulfide isomerase-like protein.
24 5837 Gma.6633 Sali3-2.
23 5593 Gma.13352 Metallothionein type 2.
22 5350 Gma.947 Lipoxygenase.
21 5107 Gma.188 Albumin 1.
17 4134 Gma.4815 Elongation factor-1A.
17 4134 Gma.27565 Seed maturation protein PM34.
15 3648 Gma.3000 Transcribed locus.
15 3648 Gma.2875 Uncharacterized LOC100306351.
14 3405 Gma.8432 Precursor (AA -24 to 403).
14 3405 Gma.30052 Peroxidase.
14 3405 Gma.22701 Class III acidic endochitinase.
14 3405 Gma.1862 Lipoxygenase-2.
13 3161 Gma.32293 Prepro-polypeptide (AA -22 to 583).
12 2918 Gma.5357 Annexin-like protein RJ4-like.
12 2918 Gma.30988 Cationic peroxidase 2.
12 2918 Gma.30728 Allergen Gly m Bd 28K.
12 2918 Gma.27475 Uncharacterized LOC100305713.
11 2675 Gma.54667 Uncharacterized LOC100796927.
11 2675 Gma.226 24 kDa oleosin isoform.
11 2675 Gma.11315 Uncharacterized LOC100819200.
10 2432 Gma.8486 Sucrose-binding protein 2.
10 2432 Gma.6921 Uncharacterized LOC100306314.
10 2432 Gma.39137 Uncharacterized LOC100500046.
9 2189 Gma.4593 Chlorophyll a-b binding protein 3, chloroplastic-like.
9 2189 Gma.4078 Uncharacterized LOC100526942.
9 2189 Gma.31106 Myo-inositol-3-phosphate synthase.
9 2189 Gma.30081 Polyubiquitin-like.
9 2189 Gma.1549 60S ribosomal protein L3-like.
8 1946 Gma.7418 Cytochrome P450 78A4-like.
8 1946 Gma.4876 Uncharacterized LOC100527166.
8 1946 Gma.31056 Seed maturation protein PM37.
8 1946 Gma.18216 Hydroxysteroid 11-beta-dehydrogenase 1-like protein-like.
8 1946 Gma.15452 60S ribosomal protein L5-like.
7 1702 Gma.7612 Gamma-interferon-inducible lysosomal thiol reductase-like.
7 1702 Gma.4707 ADP-ribosylation factor 2-like.
7 1702 Gma.414 Uncharacterized LOC100306077.
7 1702 Gma.32050 Transcribed locus, strongly similar to XP_003529049.1 PREDICTED: chaperone protein dnaJ 2-like [Glycine max].
7 1702 Gma.30725 Sali5-4a protein.
7 1702 Gma.3009 Metallothionein-II protein.
7 1702 Gma.2798 Ribulose-1,5-bisphosphate carboxylase small subunit rbcS2.
7 1702 Gma.17595 Ubiquitin-conjugation enzyme.
7 1702 Gma.14653 UDP-glucose 4-epimerase-like.
6 1459 Gma.909 Metallothionein-II protein.
6 1459 Gma.9 Dehydrin.
6 1459 Gma.6758 Transcribed locus, strongly similar to XP_003555996.1 PREDICTED: uncharacterized protein LOC100778512 [Glycine max].
6 1459 Gma.6632 LanC-like protein 2-like.
6 1459 Gma.58746 Uncharacterized LOC100306699.
6 1459 Gma.54864 60S ribosomal protein L6-like.
6 1459 Gma.54647 60S ribosomal protein L6-like.
6 1459 Gma.54644 Elongation factor 1-alpha-like.
6 1459 Gma.52559 Protease inhibitor C-II.
6 1459 Gma.4388 Aminopeptidase N-like.
6 1459 Gma.33046 Seed maturation protein PM30.
6 1459 Gma.30912 Uncharacterized LOC100306153.
6 1459 Gma.30846 S-adenosylmethionine synthase-like.
6 1459 Gma.29257 Uncharacterized LOC100527408.
6 1459 Gma.25322 Beta-conglycinin, alpha' chain-like.
6 1459 Gma.18269 Uncharacterized LOC100499824.
6 1459 Gma.15372 Uncharacterized LOC100305487.
6 1459 Gma.13660 Glyceraldehyde-3-phosphate dehydrogenase A subunit.
6 1459 Gma.12947 Chlorophyll a/b-binding protein.
6 1459 Gma.11398 ADP-ribosylation factor.
6 1459 Gma.11251 Uncharacterized LOC100306174.
5 1216 Gma.953 Uncharacterized LOC100306653.
5 1216 Gma.901 Soybean 24 kDa oleosin isoform.
5 1216 Gma.7447 Probable aquaporin PIP-type 7a-like.
5 1216 Gma.60614 Auxin-induced protein 5NG4-like.
5 1216 Gma.60613 Transcribed locus, strongly similar to NP_001242171.1 uncharacterized protein LOC100806472 [Glycine max].
5 1216 Gma.58666 Uncharacterized LOC100499804.
5 1216 Gma.5648 Adenosine 5'-phosphosulfate reductase.
5 1216 Gma.54780 Uncharacterized LOC100499771.
5 1216 Gma.54678 S-adenosylmethionine synthase-like.
5 1216 Gma.54630 Uncharacterized LOC100305687.
5 1216 Gma.5294 Uncharacterized LOC100305602.
5 1216 Gma.31779 Calnexin.
5 1216 Gma.31101 Uncharacterized LOC100818027.
5 1216 Gma.31012 40S ribosomal protein S16-like.
5 1216 Gma.30870 Sucrose synthase.
5 1216 Gma.2863 Uncharacterized LOC100305847.
5 1216 Gma.2752 COP9 signalosome complex subunit 4-like.
5 1216 Gma.20719 Kunitz trypsin protease inhibitor-like.
5 1216 Gma.1872 Sucrose-binding protein-like.
5 1216 Gma.18296 Elongation factor 1-alpha-like.
5 1216 Gma.17053 Glyceraldehyde-3-dehydrogenase C subunit.
5 1216 Gma.15766 40S ribosomal protein S19-1-like.
5 1216 Gma.1465 Ribosomal protein S11.
5 1216 Gma.11225 Uncharacterized LOC100305619.

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Source: NCBI UniGene

EST Sequences

4,376 EST sequences from this library have been submitted to the public sequence database as of 26-Feb-2012.

Source: NCBI GenBank

Click the button to save sequences to a file (FASTA format)

Keywords

  • cotyledon
  • seedling

Source: NCBI Clone Registry



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