8RE4: Cryo-EM structure of bacterial RNA polymerase-sigma54 initial transcribing complex - 5nt pre-translocated complex

PDB ID: 8RE4Download
MMDB ID: 241445
PDB Deposition Date: 2023/12/10
Updated in MMDB: 2025/07
Experimental Method:
electron microscopy
Resolution: 2.8  Å
Source Organism:
Escherichia coli K-12▼
Similar Structures:
Biological Unit for 8RE4: nonameric; determined by author
Molecular Components in 8RE4
Label Count Molecule
Proteins (6 molecules)
2
DNA-directed RNA Polymerase Subunit Alpha
Molecule annotation
1
DNA-directed RNA Polymerase Subunit Beta
Molecule annotation
1
DNA-directed RNA Polymerase Subunit Beta'
Molecule annotation
1
DNA-directed RNA Polymerase Subunit Omega
Molecule annotation
1
RNA Polymerase Sigma-54 Factor
Molecule annotation
Nucleotides(3 molecules)
1
DNA (47-mer)
Molecule annotation
1
RNA (5'-r(p*gp*cp*cp*gp*c)-3')
Molecule annotation
1
DNA (50-mer)
Molecule annotation
Chemicals and Non-standard biopolymers (3 molecules)
1
1
MAGNESIUM IONMAGNESIUM ION
2
2
ZINC IONZINC ION
* Click molecule labels to explore molecular sequence information.
Citing MMDB
Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303