8PRN: E1m, K50a, R52a, D97a, E99a Mutant Of Rh. Blastica Porin

Citation:
Abstract
The general diffusion porin from Rhodopseudomonas blastica was produced in large amounts in Escherichia coli inclusion bodies and (re)natured to the exact native structure. Here, we report on 13 mutants at the pore eyelet giving rise to new diffusion properties as measured in planar lipid bilayer experiments. The crystal structures of seven of these mutants were established. The effects of charge-modifying mutations at the pore eyelet are consistent with the known selectivity for cations. Deletions of 16 and 27 residues of the constriction loop L3 resulted in labile trimers and pores. The reduction of the eyelet cross section by introducing tryptophans gave rise to a closely correlated decrease of the conductivities. A mutant with six newly introduced tryptophans in the eyelet closed its pore in a defined manner within seconds under a voltage of 20 mV, suggesting the existence of two states. The results indicate that the pore can be engineered in a rational manner.
PDB ID: 8PRNDownload
MMDB ID: 58611
PDB Deposition Date: 1998/6/12
Updated in MMDB: 2007/10
Experimental Method:
x-ray diffraction
Resolution: 2.3  Å
Source Organism:
Similar Structures:
Biological Unit for 8PRN: trimeric; determined by author and by software (PISA,PQS)
Molecular Components in 8PRN
Label Count Molecule
Proteins (3 molecules)
3
Porin
Molecule annotation
Chemicals (9 molecules)
1
9
* Click molecule labels to explore molecular sequence information.

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