8P2I: Cryo-EM structure of Pyrococcus furiosus apo form RNA polymerase contracted clamp conformation with Spt4/5

PDB ID: 8P2IDownload
MMDB ID: 245797
PDB Deposition Date: 2023/5/16
Updated in MMDB: 2025/07
Experimental Method:
electron microscopy
Resolution: 3.4  Å
Source Organism:
Similar Structures:
Biological Unit for 8P2I: tridecameric; determined by author and by software (PISA)
Molecular Components in 8P2I
Label Count Molecule
Proteins (13 molecules)
1
DNA-directed RNA Polymerase Subunit Rpo1n
Molecule annotation
1
DNA-directed RNA Polymerase Subunit Beta
Molecule annotation
1
DNA-directed RNA Polymerase Subunit Rpo1c
(Gene symbol: rpoA2)
Molecule annotation
1
DNA-directed RNA Polymerase Subunit Rpo3
(Gene symbol: PF_RS08310)
Molecule annotation
1
DNA-directed RNA Polymerase Subunit Rpo7
Molecule annotation
1
DNA-directed RNA Polymerase Subunit Rpo4
Molecule annotation
1
DNA-directed RNA Polymerase Subunit Rpo5
(Gene symbol: PF_RS07870)
Molecule annotation
1
DNA-directed RNA Polymerase Subunit Rpo6
(Gene symbol: PF_RS08275)
Molecule annotation
1
DNA-directed RNA Polymerase Subunit Rpo11
(Gene symbol: PF_RS00250)
Molecule annotation
1
DNA-directed RNA Polymerase Subunit Rpo10
(Gene symbol: PF_RS08285)
Molecule annotation
1
DNA-directed RNA Polymerase Subunit Rpo12
(Gene symbol: PF_RS10155)
Molecule annotation
1
Transcription Elongation Factor Spt5
(Gene symbol: PF_RS10055)
Molecule annotation
1
Transcription Elongation Factor Spt4
(Gene symbol: PF_RS01290)
Molecule annotation
Chemicals and Non-standard biopolymers (7 molecules)
1
6
ZINC IONZINC ION
2
1
MAGNESIUM IONMAGNESIUM ION
* Click molecule labels to explore molecular sequence information.
Citing MMDB
Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303