8P0N: Cryo EM map and model of the vaccinia RNA polymerase intermediate pre-initiation open promoter complex shallow conformation

PDB ID: 8P0NDownload
MMDB ID: 247223
PDB Deposition Date: 2023/5/10
Updated in MMDB: 2025/07
Experimental Method:
electron microscopy
Resolution: 2.58  Å
Source Organism:
Similar Structures:
Biological Unit for 8P0N: 14-meric; determined by author
Molecular Components in 8P0N
Label Count Molecule
Proteins (12 molecules)
1
DNA-directed RNA Polymerase 147 KDA Polypeptide
(Gene symbol: J6R)
Molecule annotation
1
DNA-directed RNA Polymerase 133 KDA Polypeptide
(Gene symbol: A24R)
Molecule annotation
1
DNA-directed RNA Polymerase 35 KDA Subunit
(Gene symbol: A29L)
Molecule annotation
1
DNA-directed RNA Polymerase 22 KDA Subunit
(Gene symbol: J4R)
Molecule annotation
1
DNA-directed RNA Polymerase 19 KDA Subunit
(Gene symbol: A5R)
Molecule annotation
1
DNA-directed RNA Polymerase 18 KDA Subunit
(Gene symbol: D7R)
Molecule annotation
1
Intermediate Transcription Factor 3 Large Subunit
(Gene symbol: A23R)
Molecule annotation
1
Intermediate Transcription Factor 3 Small Subunit
(Gene symbol: A8R)
Molecule annotation
1
DNA-directed RNA Polymerase 7 KDA Subunit
(Gene symbol: G5.5R)
Molecule annotation
1
mRNA-capping Enzyme Regulatory Subunit Opg124
(Gene symbol: D12L)
Molecule annotation
1
mRNA-capping Enzyme Catalytic Subunit
(Gene symbol: D1R)
Molecule annotation
1
DNA-directed RNA Polymerase 30 KDA Polypeptide
(Gene symbol: E4L)
Molecule annotation
Nucleotides(2 molecules)
1
Non-template DNA Oligomer
Molecule annotation
1
Template DNA Oligomer
Molecule annotation
Chemicals and Non-standard biopolymers (5 molecules)
1
1
MAGNESIUM IONMAGNESIUM ION
2
4
ZINC IONZINC ION
* Click molecule labels to explore molecular sequence information.
Citing MMDB
Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303