8KAM: Crystal structure of SpyCas9 in complex with sgRNA and 16nt target DNA

PDB ID: 8KAMDownload
MMDB ID: 247847
PDB Deposition Date: 2023/8/3
Updated in MMDB: 2024/06
Experimental Method:
x-ray diffraction
Resolution: 3.91  Å
Source Organism:
Similar Structures:
Molecular Components in 8KAM
Label Count Molecule
Protein (1 molecule)
1
Crispr-associated Endonuclease Cas9/csn1
Molecule annotation
Nucleotides(3 molecules)
1
RNA (98-mer)
Molecule annotation
1
DNA (5'-d(*cp*ap*ap*tp*ap*cp*cp*tp*cp*tp*tp*cp*ap*ap*tp*tp*ap*gp*ap*ap*cp*ap*cp*g)-3')
Molecule annotation
1
DNA (5'-d(*tp*tp*tp*ap*gp*gp*tp*ap*tp*tp*g)-3')
Molecule annotation
* Click molecule labels to explore molecular sequence information.
Citing MMDB
Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303