8KAL: Crystal structure of SpyCas9 in complex with sgRNA and 17nt target DNA

PDB ID: 8KALDownload
MMDB ID: 247846
PDB Deposition Date: 2023/8/3
Updated in MMDB: 2024/06
Experimental Method:
x-ray diffraction
Resolution: 3.16  Å
Source Organism:
Similar Structures:
Biological Unit for 8KAL: tetrameric; determined by author and by software (PISA)
Molecular Components in 8KAL
Label Count Molecule
Protein (1 molecule)
1
Crispr-associated Endonuclease Cas9/csn1
Molecule annotation
Nucleotides(3 molecules)
1
RNA (98-mer)
Molecule annotation
1
DNA (25-mer)
Molecule annotation
1
DNA (5'-d(*tp*tp*tp*ap*gp*gp*tp*ap*tp*tp*g)-3')
Molecule annotation
Chemical and Non-standard biopolymers (1 molecule)
1
1
SULFATE IONSULFATE ION
* Click molecule labels to explore molecular sequence information.
Citing MMDB
Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303