5SZJ: Structure Of Human Rab10 In Complex With The Bmerb Domain Of Mical-cl

Citation:
Abstract
In their active GTP-bound form, Rab proteins interact with proteins termed effector molecules. In this study, we have thoroughly characterized a Rab effector domain that is present in proteins of the Mical and EHBP families, both known to act in endosomal trafficking. Within our study, we show that these effectors display a preference for Rab8 family proteins (Rab8, 10, 13 and 15) and that some of the effector domains can bind two Rab proteins via separate binding sites. Structural analysis allowed us to explain the specificity towards Rab8 family members and the presence of two similar Rab binding sites that must have evolved via gene duplication. This study is the first to thoroughly characterize a Rab effector protein that contains two separate Rab binding sites within a single domain, allowing Micals and EHBPs to bind two Rabs simultaneously, thus suggesting previously unknown functions of these effector molecules in endosomal trafficking.
PDB ID: 5SZJDownload
MMDB ID: 142367
PDB Deposition Date: 2016/8/14
Updated in MMDB: 2017/09
Experimental Method:
x-ray diffraction
Resolution: 2.66  Å
Source Organism:
Similar Structures:
Biological Unit for 5SZJ: dimeric; determined by software (PISA)
Molecular Components in 5SZJ
Label Count Molecule
Proteins (2 molecules)
1
Ras-related Protein Rab-10(Gene symbol: RAB10)
Molecule annotation
1
Mical C-terminal-like Protein(Gene symbol: MICALCL)
Molecule annotation
Chemicals (3 molecules)
1
1
2
1
3
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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