5SWZ: Crystal Structure Of Np1-b17 Tcr-h2db-np Complex

Citation:
Abstract
The anti-viral T cell response is drawn from the naive T cell repertoire. During influenza infection, the CD8+ T cell response to an H-2Db-restricted nucleoprotein epitope (NP366) is characterized by preferential expansion of T cells bearing TRBV13+ T cell receptors (TCRs) and avoidance of TRBV17+ T cells, despite the latter dominating the naive precursor repertoire. We found two TRBV17+ TCRs that bound H-2Db-NP366 with a 180 degrees reversed polarity compared to the canonical TCR-pMHC-I docking. The TRBV17 beta-chain dominated the interaction and, whereas the complementarity determining region-3 (CDR3) loops exclusively mediated contacts with the MHC-I, peptide specificity was attributable to germline-encoded recognition. Nevertheless, the TRBV17+ TCR exhibited moderate affinity toward H-2Db-NP366 and was capable of signal transduction. Thus, the naive CD8+ T cell pool can comprise TCRs adopting reversed pMHC-I docking modes that limit their involvement in the immune response.
PDB ID: 5SWZDownload
MMDB ID: 143858
PDB Deposition Date: 2016/8/9
Updated in MMDB: 2016/10
Experimental Method:
x-ray diffraction
Resolution: 2.65  Å
Source Organism:
unidentified influenza virus
Similar Structures:
Biological Unit for 5SWZ: pentameric; determined by author
Molecular Components in 5SWZ
Label Count Molecule
Proteins (5 molecules)
1
H-2 Class I Histocompatibility Antigen, D-B Alpha Chain(Gene symbol: H2-D1)
Molecule annotation
1
Beta-2-microglobulin(Gene symbol: B2m)
Molecule annotation
1
Influenza Np366 Epitope
Molecule annotation
1
Np1-b17 TCR Alpha Chain
Molecule annotation
1
Np1-b17 TCR Beta Chain
Molecule annotation
Chemicals (3 molecules)
1
2
2
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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