5NFV: Crystal structure of catalytically inactive FnCas12 mutant bound to an R-loop structure containing a pre-crRNA mimic and full-length DNA target

PDB ID: 5NFVDownload
MMDB ID: 151735
PDB Deposition Date: 2017/3/16
Updated in MMDB: 2024/10
Experimental Method:
x-ray diffraction
Resolution: 2.501  Å
Source Organism:
Francisella tularensis subsp. novicida U112▼
Similar Structures:
Biological Unit for 5NFV: tetrameric; determined by software (PISA)
Molecular Components in 5NFV
Label Count Molecule
Protein (1 molecule)
1
Crispr-associated Endonuclease Cpf1
Molecule annotation
Nucleotides(3 molecules)
1
Pre-crrna
Molecule annotation
1
DNA Target Strand
Molecule annotation
1
DNA Non-target Strand
Molecule annotation
Chemicals and Non-standard biopolymers (4 molecules)
1
3
MAGNESIUM IONMAGNESIUM ION
2
1
2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL
* Click molecule labels to explore molecular sequence information.
Citing MMDB
Madej T, Lanczycki CJ, Zhang D, Thiessen PA, Geer RC, Marchler-Bauer A, Bryant SH. " MMDB and VAST+: tracking structural similarities between macromolecular complexes. Nucleic Acids Res. 2014 Jan; 42(Database issue):D297-303