5MD4: The Structure Of The Mature Hiv-1 Ca Hexameric Lattice With Curvature Parameters: Tilt=11, Twist=6

Citation:
Abstract
HIV-1 contains a cone-shaped capsid encasing the viral genome. This capsid is thought to follow fullerene geometry-a curved hexameric lattice of the capsid protein, CA, closed by incorporating 12 CA pentamers. Current models for core structure are based on crystallography of hexameric and cross-linked pentameric CA, electron microscopy of tubular CA arrays, and simulations. Here, we report subnanometer-resolution cryo-electron tomography structures of hexameric and pentameric CA within intact HIV-1 particles. Whereas the hexamer structure is compatible with crystallography studies, the pentamer forms using different interfaces. Determining multiple structures revealed how CA flexes to form the variably curved core shell. We show that HIV-1 CA assembles both aberrant and perfect fullerene cones, supporting models in which conical cores assemble de novo after maturation.
PDB ID: 5MD4Download
MMDB ID: 146021
PDB Deposition Date: 2016/11/10
Updated in MMDB: 2016/12
Experimental Method:
electron microscopy
Resolution: 8.4  Å
Source Organism:
Similar Structures:
Biological Unit for 5MD4: tetradecameric; determined by author
Molecular Components in 5MD4
Label Count Molecule
Proteins (14 molecules)
8
Capsid Protein P24
Molecule annotation
6
Capsid Protein P24
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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