5LNC: Structure Of Spx Domain Of The Yeast Inorganic Polyphophate Polymerase Vtc4 Crystallized By Carrier-driven Crystallization In Fusion With The Macro Domain Of Human Histone Macroh2a1.1

Obtaining well-ordered crystals remains a significant challenge in protein X-ray crystallography. Carrier-driven crystallization can facilitate crystal formation and structure solution of difficult target proteins. We obtained crystals of the small and highly flexible SPX domain from the yeast vacuolar transporter chaperone 4 (Vtc4) when fused to a C-terminal, non-cleavable macro tag derived from human histone macroH2A1.1. Initial crystals diffracted to 3.3 A resolution. Reductive protein methylation of the fusion protein yielded a new crystal form diffracting to 2.1 A. The structures were solved by molecular replacement, using isolated macro domain structures as search models. Our findings suggest that macro domain tags can be employed in recombinant protein expression in E. coli, and in carrier-driven crystallization.
PDB ID: 5LNCDownload
MMDB ID: 144827
PDB Deposition Date: 2016/8/3
Updated in MMDB: 2016/11
Experimental Method:
x-ray diffraction
Resolution: 3.29  Å
Similar Structures:
Biological Unit for 5LNC: monomeric; determined by author
Molecular Components in 5LNC
Label Count Molecule
Protein (1 molecule)
Vacuolar Transporter Chaperone 4,core Histone Macro-h2a.1
Molecule annotation
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Citing MMDB