5KTY: Hmiro Ef Hand And Cgtpase Domains, Gdp And Ca2+ Bound State

Citation:
Abstract
Hereditary Parkinson's disease is commonly caused by mutations in the protein kinase PINK1 or the E3 ubiquitin ligase Parkin, which function together to eliminate damaged mitochondria. PINK1 phosphorylates both Parkin and ubiquitin to stimulate ubiquitination of dozens of proteins on the surface of the outer mitochondrial membrane. However, the mechanisms by which Parkin recognizes specific proteins for modification remain largely unexplored. Here, we show that the C-terminal GTPase (cGTPase) of the Parkin primary substrate human Miro is necessary and sufficient for efficient ubiquitination. We present several new X-ray crystal structures of both human Miro1 and Miro2 that reveal substrate recognition and ubiquitin transfer to be specific to particular protein domains and lysine residues. We also provide evidence that Parkin substrate recognition is functionally separate from substrate modification. Finally, we show that prioritization for modification of a specific lysine sidechain of the cGTPase (K572) within human Miro1 is dependent on both its location and chemical microenvironment. Activation of Parkin by phosphorylation or by binding of pUb is required for prioritization of K572 for modification, suggesting that Parkin activation and acquisition of substrate specificity are coupled.
PDB ID: 5KTYDownload
MMDB ID: 143356
PDB Deposition Date: 2016/7/12
Updated in MMDB: 2016/09
Experimental Method:
x-ray diffraction
Resolution: 2.52  Å
Source Organism:
Similar Structures:
Biological Unit for 5KTY: monomeric; determined by author and by software (PISA)
Molecular Components in 5KTY
Label Count Molecule
Protein (1 molecule)
1
Mitochondrial RHO Gtpase 1(Gene symbol: RHOT1)
Molecule annotation
Chemicals (5 molecules)
1
2
2
1
3
2
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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