5KBW: Crystal structure of TmRibU, the riboflavin-binding S subunit from the Thermotoga maritima ECF transporter

Citation:
Abstract
ECF transporters are a family of active membrane transporters for essential micronutrients, such as vitamins and trace metals. Found exclusively in archaea and bacteria, these transporters are composed of four subunits: an integral membrane substrate-binding subunit (EcfS), a transmembrane coupling subunit (EcfT), and two ATP-binding cassette ATPases (EcfA and EcfA'). We have characterized the structural basis of substrate binding by the EcfS subunit for riboflavin from Thermotoga maritima, TmRibU. TmRibU binds riboflavin with high affinity, and the protein-substrate complex is exceptionally stable in solution. The crystal structure of riboflavin-bound TmRibU reveals an electronegative binding pocket at the extracellular surface in which the substrate is completely buried. Analysis of the intermolecular contacts indicates that nearly every available substrate hydrogen bond is satisfied. A conserved aromatic residue at the extracellular end of TM5, Tyr130, caps the binding site to generate a substrate-bound, occluded state, and non-conservative mutation of Tyr130 reduces the stability of this conformation. Using a novel fluorescence binding assay, we find that an aromatic residue at this position is essential for high-affinity substrate binding. Comparison with other S subunit structures suggests that TM5 and Loop5-6 contain a dynamic, conserved motif that plays a key role in gating substrate entry and release by S subunits of ECF transporters.
PDB ID: 5KBWDownload
MMDB ID: 140592
PDB Deposition Date: 2016/6/3
Updated in MMDB: 2017/11
Experimental Method:
x-ray diffraction
Resolution: 2.6093  Å
Source Organism:
Similar Structures:
Biological Unit for 5KBW: monomeric; determined by author
Molecular Components in 5KBW
Label Count Molecule
Protein (1 molecule)
1
Riboflavin Transporter Ribu(Gene symbol: TM1455)
Molecule annotation
Chemical (1 molecule)
1
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.