5K5B: Wild-type Pas-gaf Fragment From Deinococcus Radiodurans Bphp

Citation:
Abstract
Phytochromes are a family of photoreceptors that control light responses of plants, fungi and bacteria. A sequence of structural changes, which is not yet fully understood, leads to activation of an output domain. Time-resolved serial femtosecond crystallography (SFX) can potentially shine light on these conformational changes. Here we report the room temperature crystal structure of the chromophore-binding domains of the Deinococcus radiodurans phytochrome at 2.1 A resolution. The structure was obtained by serial femtosecond X-ray crystallography from microcrystals at an X-ray free electron laser. We find overall good agreement compared to a crystal structure at 1.35 A resolution derived from conventional crystallography at cryogenic temperatures, which we also report here. The thioether linkage between chromophore and protein is subject to positional ambiguity at the synchrotron, but is fully resolved with SFX. The study paves the way for time-resolved structural investigations of the phytochrome photocycle with time-resolved SFX.
PDB ID: 5K5BDownload
MMDB ID: 144390
PDB Deposition Date: 2016/5/23
Updated in MMDB: 2016/11
Experimental Method:
x-ray diffraction
Resolution: 1.35  Å
Source Organism:
Similar Structures:
Biological Unit for 5K5B: dimeric; determined by software (PISA)
Molecular Components in 5K5B
Label Count Molecule
Proteins (2 molecules)
2
Bacteriophytochrome(Gene symbol: DR_A0050)
Molecule annotation
Chemicals (9 molecules)
1
6
2
1
3
2
* Click molecule labels to explore molecular sequence information.

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