National Center for
5JHM: Crystal Structure Of Zika Virus Envelope Protein
Structures of the Zika Virus Envelope Protein and Its Complex with a Flavivirus Broadly Protective Antibody
Cell Host Microbe (2016) 19 p.696-704
Zika virus (ZIKV), a mosquito-borne flavivirus, is a current global public health concern. The flavivirus envelope (E) glycoprotein is responsible for virus entry and represents a major target of neutralizing antibodies for other flaviviruses. Here, we report the structures of ZIKV E protein at 2.0 A and in complex with a flavivirus broadly neutralizing murine antibody 2A10G6 at 3.0 A. ZIKV-E resembles all the known flavivirus E structures but contains a unique, positively charged patch adjacent to the fusion loop region of the juxtaposed monomer, which may influence host attachment. The ZIKV-E-2A10G6 complex structure reveals antibody recognition of a highly conserved fusion loop. 2A10G6 binds to ZIKV-E with high affinity in vitro and neutralizes currently circulating ZIKV strains in vitro and in mice. The E protein fusion loop epitope represents a potential candidate for therapeutic antibodies against ZIKV.