5JHI: Solution structure of the de novo mini protein gEHE_06

Citation:
Abstract
Naturally occurring, pharmacologically active peptides constrained with covalent crosslinks generally have shapes that have evolved to fit precisely into binding pockets on their targets. Such peptides can have excellent pharmaceutical properties, combining the stability and tissue penetration of small-molecule drugs with the specificity of much larger protein therapeutics. The ability to design constrained peptides with precisely specified tertiary structures would enable the design of shape-complementary inhibitors of arbitrary targets. Here we describe the development of computational methods for accurate de novo design of conformationally restricted peptides, and the use of these methods to design 18-47 residue, disulfide-crosslinked peptides, a subset of which are heterochiral and/or N-C backbone-cyclized. Both genetically encodable and non-canonical peptides are exceptionally stable to thermal and chemical denaturation, and 12 experimentally determined X-ray and NMR structures are nearly identical to the computational design models. The computational design methods and stable scaffolds presented here provide the basis for development of a new generation of peptide-based drugs.
PDB ID: 5JHIDownload
MMDB ID: 143549
PDB Deposition Date: 2016/4/21
Updated in MMDB: 2016/11
Experimental Method:
solution nmr
Source Organism:
Similar Structures:
Biological Unit for 5JHI: monomeric; determined by author
Molecular Components in 5JHI
Label Count Molecule
Protein (1 molecule)
1
W35
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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