5J8Q: Crystal Structure Of The Cysteine Desulfurase Sufs Of Bacillus Subtilis

The biosynthesis of iron sulfur (Fe-S) clusters in Bacillus subtilis is mediated by a SUF-type gene cluster, consisting of the cysteine desulfurase SufS, the scaffold protein SufU, and the putative chaperone complex SufB/SufC/SufD. Here, we present the high-resolution crystal structure of the SufS homodimer in its product-bound state (i.e., in complex with pyrodoxal-5'-phosphate, alanine, Cys361-persulfide). By performing hydrogen/deuterium exchange (H/DX) experiments, we characterized the interaction of SufS with SufU and demonstrate that SufU induces an opening of the active site pocket of SufS. Recent data indicate that frataxin could be involved in Fe-S cluster biosynthesis by facilitating iron incorporation. H/DX experiments show that frataxin indeed interacts with the SufS/SufU complex at the active site. Our findings deepen the current understanding of Fe-S cluster biosynthesis, a complex yet essential process, in the model organism B. subtilis.
PDB ID: 5J8QDownload
MMDB ID: 141384
PDB Deposition Date: 2016/4/8
Updated in MMDB: 2016/08
Experimental Method:
x-ray diffraction
Resolution: 1.7  Å
Source Organism:
Similar Structures:
Biological Unit for 5J8Q: dimeric; determined by author and by software (PISA)
Molecular Components in 5J8Q
Label Count Molecule
Proteins (2 molecules)
Cysteine Desulfurase Sufs(Gene symbol: sufS)
Molecule annotation
Chemicals (4 molecules)
* Click molecule labels to explore molecular sequence information.

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