5J76: Structure Of Lectin From Colocasia Esculenta(l.) Schott

Citation:
Abstract
The Mannose-binding beta-Prism Colocasia esculenta lectin (beta-PCL) was purified from tubers using ion exchange chromatography. The purified beta-PCL appeared as a single band of approximately 12kDa on SDS-PAGE. beta-PCL crystallizes in trigonal space group P3121 and diffracted to a resolution of 2.1A. The structure was solved using Molecular replacement using Crocus vernus lectin (PDB: 3MEZ) as a model. From the final refined model to an R-factor of 16.5% and an Rfree of 20.4%, it has been observed that the biological unit consists of two beta-Prism domains augmented through C-terminals swap over to form one of faces for each domain. Calpha superposition of individual domains of beta-PCL with individual domains of other related structures and superposition of whole protein structures were carried out. The higher RMS deviation for the superposition of whole structures suggest that beta-prism domains assume different orientation in each structure.
PDB ID: 5J76Download
MMDB ID: 140304
PDB Deposition Date: 2016/4/6
Updated in MMDB: 2016/07
Experimental Method:
x-ray diffraction
Resolution: 2.1  Å
Source Organism:
Similar Structures:
Biological Unit for 5J76: dimeric; determined by author and by software (PISA)
Molecular Components in 5J76
Label Count Molecule
Proteins (2 molecules)
1
12kd Storage Protein
Molecule annotation
1
12kd Storage Protein
Molecule annotation
Chemicals (2 molecules)
1
2
* Click molecule labels to explore molecular sequence information.

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