5J3R: Crystal Structure Of Yeast Monothiol Glutaredoxin Grx6 In Complex With A Glutathione-coordinated [2fe-2s] Cluster

Citation:
Abstract
Glutaredoxins (Grxs) constitute a superfamily of proteins that perform diverse biological functions. The Saccharomyces cerevisiae glutaredoxin Grx6 not only serves as a glutathione (GSH)-dependent oxidoreductase and as a GSH transferase, but also as an essential [2Fe-2S]-binding protein. Here, the dimeric structure of the C-terminal domain of Grx6 (holo Grx6C), bridged by one [2Fe-2S] cluster coordinated by the active-site Cys136 and two external GSH molecules, is reported. Structural comparison combined with multiple-sequence alignment demonstrated that holo Grx6C is similar to the [2Fe-2S] cluster-incorporated dithiol Grxs, which share a highly conserved [2Fe-2S] cluster-binding pattern and dimeric conformation that is distinct from the previously identified [2Fe-2S] cluster-ligated monothiol Grxs.
PDB ID: 5J3RDownload
MMDB ID: 144136
PDB Deposition Date: 2016/3/31
Updated in MMDB: 2016/10
Experimental Method:
x-ray diffraction
Resolution: 2.46  Å
Source Organism:
Similar Structures:
Biological Unit for 5J3R: dimeric; determined by author and by software (PISA)
Molecular Components in 5J3R
Label Count Molecule
Proteins (2 molecules)
2
Monothiol Glutaredoxin-6(Gene symbol: GRX6)
Molecule annotation
Chemicals (3 molecules)
1
1
2
2
* Click molecule labels to explore molecular sequence information.

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