5IUZ: Structure Of P450 2b4 F202w Mutant (cymal-5)

Multiple crystal structures of CYP2B4 have demonstrated the binding of the detergent 5-cyclohexyl-1-pentyl-beta-D-maltoside (CYMAL-5) in a peripheral pocket located adjacent to the active site. To explore the consequences of detergent binding, X-ray crystal structures of the peripheral pocket mutant CYP2B4 F202W were solved in the presence of hexaethylene glycol monooctyl ether (C8E6) and CYMAL-5. The structure in the presence of CYMAL-5 illustrated a closed conformation indistinguishable from the previously solved wild-type. In contrast, the F202W structure in the presence of C8E6 revealed a detergent molecule that coordinated the heme-iron and extended to the protein surface through the substrate access channel 2f. Despite the overall structural similarity of these detergent complexes, remarkable differences were observed in the A, A', and H helices, the F-G cassette, the C-D and beta4 loop region. Hydrogen-deuterium exchange mass spectrometry (DXMS) was employed to probe these differences and to test the effect of detergents in solution. The presence of either detergent increased the H/D exchange rate across the plastic regions, and the results obtained by DXMS in solution were consistent in general with the relevant structural snapshots. The study provides insight into effect of detergent binding and the interpretation of associated conformational dynamics of CYP2B4.
PDB ID: 5IUZDownload
MMDB ID: 139971
PDB Deposition Date: 2016/3/18
Updated in MMDB: 2016/06
Experimental Method:
x-ray diffraction
Resolution: 2.73  Å
Source Organism:
Similar Structures:
Biological Unit for 5IUZ: monomeric; determined by author and by software (PISA)
Molecular Components in 5IUZ
Label Count Molecule
Protein (1 molecule)
Cytochrome P450 2B4(Gene symbol: CYP2B4)
Molecule annotation
Chemicals (3 molecules)
* Click molecule labels to explore molecular sequence information.

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