5HYQ: Cetuximab Fab in complex with amidated meditope

Citation:
Abstract
Recently, a unique binding site for a cyclic 12-residue peptide was discovered within a cavity formed by the light and heavy chains of the cetuximab Fab domain. In order to better understand the interactions that drive this unique complex, a number of variants including the residues within the meditope peptide and the antibody, as well as the cyclization region of the meditope peptide, were created. Here, multiple crystal structures of meditope peptides incorporating different cyclization strategies bound to the central cavity of the cetuximab Fab domain are presented. The affinity of each cyclic derivative for the Fab was determined by surface plasmon resonance and correlated to structural differences. Overall, it was observed that the disulfide bond used to cyclize the peptide favorably packs against a hydrophobic `pocket' and that amidation and acetylation of the original disulfide meditope increased the overall affinity approximately 2.3-fold. Conversely, replacing the terminal cysteines with serines and thus creating a linear peptide reduced the affinity over 50-fold, with much of this difference being reflected in a decrease in the on-rate. Other cyclization methods, including the formation of a lactam, reduced the affinity but not to the extent of the linear peptide. Collectively, the structural and kinetic data presented here indicate that small perturbations introduced by different cyclization strategies can significantly affect the affinity of the meditope-Fab complex.
PDB ID: 5HYQDownload
MMDB ID: 140108
PDB Deposition Date: 2016/2/1
Updated in MMDB: 2016/06
Experimental Method:
x-ray diffraction
Resolution: 2.477  Å
Similar Structures:
Biological Unit for 5HYQ: trimeric; determined by author and by software (PISA)
Molecular Components in 5HYQ
Label Count Molecule
Proteins (3 molecules)
1
Cetuximab Light Chain
Molecule annotation
1
Cetuximab Heavy Chain
Molecule annotation
1
Amidated Meditope
Molecule annotation
Chemical (1 molecule)
1
1
* Click molecule labels to explore molecular sequence information.

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