5HHZ: Crystal Structure Of Maize Cytokinin Oxidase/dehydrogenase 4 (zmcko4) In Complex With 6-(3-methylpyrrol-1-yl)-9h-purine

Cytokinins (CKs) are an important group of phytohormones. Their tightly regulated and balanced levels are essential for proper cell division and plant organ development. Here we report precise quantification of CK metabolites and other phytohormones in maize reproductive organs in the course of pollination and kernel maturation. A novel enzymatic activity dependent on NADP(+) converting trans-zeatin (tZ) to 6-(3-methylpyrrol-1-yl)purine (MPP) was detected. MPP shows weak anticytokinin properties and inhibition of CK dehydrogenases due to their ability to bind to an active site in the opposite orientation than substrates. Although the physiological significance of tZ side-chain cyclization is not anticipated as the MPP occurrence in maize tissue is very low, properties of the novel CK metabolite indicate its potential for utilization in plant in vitro tissue culture. Furthermore, feeding experiments with different isoprenoid CKs revealed distinct preferences in glycosylation of tZ and cis-zeatin (cZ). While tZ is preferentially glucosylated at the N9 position, cZ forms mainly O-glucosides. Since O-glucosides, in contrast to N9-glucosides, are resistant to irreversible cleavage catalyzed by CK dehydrogenases, the observed preference of maize CK glycosyltransferases to O-glycosylate zeatin in the cis-position might be a reason why cZ derivatives are over-accumulated in different maize tissues and organs.
PDB ID: 5HHZDownload
MMDB ID: 138968
PDB Deposition Date: 2016/1/11
Updated in MMDB: 2016/05
Experimental Method:
x-ray diffraction
Resolution: 2  Å
Source Organism:
Similar Structures:
Biological Unit for 5HHZ: monomeric; determined by author and by software (PISA)
Molecular Components in 5HHZ
Label Count Molecule
Protein (1 molecule)
Cytokinin Dehydrogenase 4
Molecule annotation
Chemicals (2 molecules)
* Click molecule labels to explore molecular sequence information.

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