5HBC: Intermediate Structure Of Iron-saturated C-lobe Of Bovine Lactoferrin At 2.79 Angstrom Resolution Indicates The Softening Of Iron Coordination

Citation:
Abstract
The bilobal lactoferrin is an approximately 76 kDa glycoprotein. It sequesters two Fe(3+) ions together with two CO32- ions. The C-terminal half (residues, Tyr342-Arg689, C-lobe) of bovine lactoferrin (BLF) (residues Ala1-Arg689) was prepared by limited proteolysis using trypsin. Both C-lobe and intact BLF were saturated to 100%. Both of them retained up to nearly 85% of iron at pH 6.5. At pH 5.0, C-lobe retained 75% of iron whereas intact protein could retain only slightly more than 60%. At pH 4.0 both contained 25% iron and at pH 2.0 they were left with iron concentration of only 10%. The structure of iron saturated C-lobe was determined at 2.79 A resolution and refined to Rcryst and Rfree factors of 0.205 and 0.273, respectively. The structure contains two crystallographically independent molecules, A and B. They were found to have identical structures with an r.m.s. shift of 0.5 A for their C(alpha) atoms. A high solvent content of 66% was observed in the crystals. The average value of an overall B-factor was 68.0 A(2) . The distance of 2.9 A observed for the coordination bond between Fe(3+) ion and N(e2) of His595 appeared to be considerably longer than the normally observed values of 1.9-2.2 A. This indicated that the coordination bond involving His595 may be absent. Other coordination distances were observed in the range of 2.1-2.3 A. Based on the present structure of iron saturated C-lobe, it may be stated that His595 is the first residue to dissociate from ferric ion when the pH is lowered. Proteins 2016; 84:591-599. (c) 2016 Wiley Periodicals, Inc.
PDB ID: 5HBCDownload
MMDB ID: 135843
PDB Deposition Date: 2015/12/31
Updated in MMDB: 2016/07
Experimental Method:
x-ray diffraction
Resolution: 2.79  Å
Source Organism:
Similar Structures:
Biological Unit for 5HBC: monomeric; determined by author and by software (PISA)
Molecular Components in 5HBC
Label Count Molecule
Protein (1 molecule)
1
Lactotransferrin(Gene symbol: LTF)
Molecule annotation
Chemicals (6 molecules)
1
4
2
1
3
1
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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