5GSP: Ribonuclease T13'-Gmp, 9 Weeks

Here we present a time-resolved crystallographic analysis of the hydrolysis of exo (Sp) guanosine 2',3'-cyclophosphorothioate by RNase T1. The use of a slow substrate and fast crystallization methods made it possible to perform the study with conventional data-collection techniques. The results support the idea that the hydrolysis reaction proceeds through a mechanism that is the inverse of the transesterification reaction. In addition, the structures provide an explanation for the differential behavior of RNase T1 towards exo- and endo-cyclic thiophosphates.
PDB ID: 5GSPDownload
MMDB ID: 58496
PDB Deposition Date: 1997/12/9
Updated in MMDB: 2012/10
Experimental Method:
x-ray diffraction
Resolution: 1.8  Å
Source Organism:
Similar Structures:
Biological Unit for 5GSP: monomeric; determined by author
Molecular Components in 5GSP
Label Count Molecule
Protein (1 molecule)
Ribonuclease T1(Gene symbol: AO090011000118)
Molecule annotation
Chemicals (2 molecules)
* Click molecule labels to explore molecular sequence information.

Citing MMDB