5G2Q: The Crystal Structure Of A S-selective Transaminase From Arthrobacter Sp. With Alanine Bound

Citation:
Abstract
omega-Transaminases are enzymes that can introduce an amino group in industrially interesting compounds. We determined crystal structures of two (S)-selective omega-transaminases, one from Arthrobacter sp. (Ars-omegaTA) and one from Bacillus megaterium (BM-omegaTA), which have 95% identical sequences but somewhat different activity profiles. Substrate profiling measurements using a range of (R)- and (S)-substrates showed that both enzymes have a preference for substrates with large, flat cyclic side groups, for which the activity of BM-omegaTA is generally somewhat higher. BM-omegaTA has a preference for (S)-3,3-dimethyl-2-butylamine significantly stronger than that of Ars-omegaTA, as well as a weaker enantiopreference for 1-cyclopropylethylamine. The crystal structures showed that, as expected for (S)-selective transaminases, both enzymes have the typical transaminase type I fold and have spacious active sites to accommodate largish substrates. A structure of BM-omegaTA with bound (R)-alpha-methylbenzylamine explains the enzymes' preference for (S)-substrates. Site-directed mutagenesis experiments revealed that the presence of a tyrosine, instead of a cysteine, at position 60 increases the relative activities on several small substrates. A structure of Ars-omegaTA with bound l-Ala revealed that the Arg442 side chain has been repositioned to bind the l-Ala carboxylate. Compared to the arginine switch residue in other transaminases, Arg442 is shifted by six residues in the amino acid sequence, which appears to be a consequence of extra loops near the active site that narrow the entrance to the active site.
PDB ID: 5G2QDownload
MMDB ID: 141333
PDB Deposition Date: 2016/4/12
Updated in MMDB: 2016/08
Experimental Method:
x-ray diffraction
Resolution: 2.3  Å
Source Organism:
Similar Structures:
Biological Unit for 5G2Q: tetrameric; determined by author and by software (PISA)
Molecular Components in 5G2Q
Label Count Molecule
Proteins (4 molecules)
4
Transaminase
Molecule annotation
Chemicals (4 molecules)
1
4
* Click molecule labels to explore molecular sequence information.

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