5FYL: Crystal Structure At 3.7 A Resolution Of Fully Glycosylated Hiv-1 Clade A Bg505 Sosip.664 Prefusion Env Trimer In Complex With Broadly Neutralizing Antibodies Pgt122 And 35o22

Citation:
Abstract
The HIV-1-envelope (Env) trimer is covered by a glycan shield of approximately 90 N-linked oligosaccharides, which comprises roughly half its mass and is a key component of HIV evasion from humoral immunity. To understand how antibodies can overcome the barriers imposed by the glycan shield, we crystallized fully glycosylated Env trimers from clades A, B, and G, visualizing the shield at 3.4-3.7 A resolution. These structures reveal the HIV-1-glycan shield to comprise a network of interlocking oligosaccharides, substantially ordered by glycan crowding, that encase the protein component of Env and enable HIV-1 to avoid most antibody-mediated neutralization. The revealed features delineate a taxonomy of N-linked glycan-glycan interactions. Crowded and dispersed glycans are differently ordered, conserved, processed, and recognized by antibody. The structures, along with glycan-array binding and molecular dynamics, reveal a diversity in oligosaccharide affinity and a requirement for accommodating glycans among known broadly neutralizing antibodies that target the glycan-shielded trimer.
PDB ID: 5FYLDownload
MMDB ID: 140881
PDB Deposition Date: 2016/3/8
Updated in MMDB: 2016/08
Experimental Method:
x-ray diffraction
Resolution: 3.1  Å
Source Organism:
Human immunodeficiency virus 1
Similar Structures:
Biological Unit for 5FYL: octadecameric; determined by software (PISA)
Molecular Components in 5FYL
Label Count Molecule
Proteins (18 molecules)
3
Bg505 Gp120 ENV Ectodomain
Molecule annotation
3
35o22 Antibody FAB Heavy Chain
Molecule annotation
3
35o22 Antibody FAB Light Chain
Molecule annotation
3
Bg505 Gp120 ENV Ectodomain
Molecule annotation
3
Pgt122 Antibody FAB Heavy Chain
Molecule annotation
3
Pgt122 Antibody FAB Light Chain
Molecule annotation
Chemicals (144 molecules)
1
87
2
15
3
42
* Click molecule labels to explore molecular sequence information.

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