5FXN: Structure of thermolysin solved by SAD from data collected by Direct Data Collection (DDC) using the ESRF RoboDiff goniometer

Citation:
Abstract
Automation of the mounting of cryocooled samples is now a feature of the majority of beamlines dedicated to macromolecular crystallography (MX). Robotic sample changers have been developed over many years, with the latest designs increasing capacity, reliability and speed. Here, the development of a new sample changer deployed at the ESRF beamline MASSIF-1 (ID30A-1), based on an industrial six-axis robot, is described. The device, named RoboDiff, includes a high-capacity dewar, acts as both a sample changer and a high-accuracy goniometer, and has been designed for completely unattended sample mounting and diffraction data collection. This aim has been achieved using a high level of diagnostics at all steps of the process from mounting and characterization to data collection. The RoboDiff has been in service on the fully automated endstation MASSIF-1 at the ESRF since September 2014 and, at the time of writing, has processed more than 20 000 samples completely automatically.
PDB ID: 5FXNDownload
MMDB ID: 137525
PDB Deposition Date: 2016/3/2
Updated in MMDB: 2017/09
Experimental Method:
x-ray diffraction
Resolution: 1.45  Å
Source Organism:
Similar Structures:
Biological Unit for 5FXN: monomeric; determined by author and by software (PISA)
Molecular Components in 5FXN
Label Count Molecule
Protein (1 molecule)
1
Thermolysin
Molecule annotation
Chemicals (7 molecules)
1
1
2
1
3
1
4
4
* Click molecule labels to explore molecular sequence information.

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