5FOW: Humanised Monomeric Rada In Complex With Whta Tetrapeptide

Citation:
Abstract
RAD51 is a recombinase involved in the homologous recombination of double-strand breaks in DNA. RAD51 forms oligomers by binding to another molecule of RAD51 via an 'FxxA' motif, and the same recognition sequence is similarly utilised to bind BRCA2. We have tabulated the effects of mutation of this sequence, across a variety of experimental methods and from relevant mutations observed in the clinic. We use mutants of a tetrapeptide sequence to probe the binding interaction, using both isothermal titration calorimetry and X-ray crystallography. Where possible, comparison between our tetrapeptide mutational study and the previously reported mutations is made, discrepancies are discussed and the importance of secondary structure in interpreting alanine scanning and mutational data of this nature is considered.
PDB ID: 5FOWDownload
MMDB ID: 137944
PDB Deposition Date: 2015/11/26
Updated in MMDB: 2017/07
Experimental Method:
x-ray diffraction
Resolution: 1.8  Å
Source Organism:
Homo sapiens
Similar Structures:
Biological Unit for 5FOW: dimeric; determined by author and by software (PISA)
Molecular Components in 5FOW
Label Count Molecule
Proteins (2 molecules)
1
UNKNOWN(Gene symbol: PF_RS09730)
Molecule annotation
1
Whta Peptide
Molecule annotation
Chemicals (2 molecules)
1
1
2
1
* Click molecule labels to explore molecular sequence information.

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