5FN1: Electron cryo-microscopy of filamentous flexible virus PepMV (Pepino Mosaic Virus)

Citation:
Abstract
Flexible filamentous viruses include economically important plant pathogens. Their viral particles contain several hundred copies of a helically arrayed coat protein (CP) protecting a (+)ssRNA. We describe here a structure at 3.9 A resolution, from electron cryomicroscopy, of Pepino mosaic virus (PepMV), a representative of the genus Potexvirus (family Alphaflexiviridae). Our results allow modeling of the CP and its interactions with viral RNA. The overall fold of PepMV CP resembles that of nucleoproteins (NPs) from the genus Phlebovirus (family Bunyaviridae), a group of enveloped (-)ssRNA viruses. The main difference between potexvirus CP and phlebovirus NP is in their C-terminal extensions, which appear to determine the characteristics of the distinct multimeric assemblies - a flexuous, helical rod or a loose ribonucleoprotein. The homology suggests gene transfer between eukaryotic (+) and (-)ssRNA viruses.
PDB ID: 5FN1Download
MMDB ID: 135246
PDB Deposition Date: 2015/11/10
Updated in MMDB: 2016/12
Experimental Method:
electron microscopy
Resolution: 3.9  Å
Source Organism:
Similar Structures:
Biological Unit for 5FN1: 36-meric
Molecular Components in 5FN1
Label Count Molecule
Proteins (18 molecules)
18
Coat Protein
Molecule annotation
Nucleotide(1 molecule)
18
5'-r(*up*up*up*up*up)-3'
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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