5FJ5: Structure Of The In Vitro Assembled Bacteriophage Phi6 Polymerase Complex

Electron cryomicroscopy can yield near-atomic resolution structures of highly ordered macromolecular complexes. Often however some subunits bind in a flexible manner, have different symmetry from the rest of the complex, or are present in sub-stoichiometric amounts, limiting the attainable resolution. Here we report a general method for the localized three-dimensional reconstruction of such subunits. After determining the particle orientations, local areas corresponding to the subunits can be extracted and treated as single particles. We demonstrate the method using three examples including a flexible assembly and complexes harbouring subunits with either partial occupancy or mismatched symmetry. Most notably, the method allows accurate fitting of the monomeric RNA-dependent RNA polymerase bound at the threefold axis of symmetry inside a viral capsid, revealing for the first time its exact orientation and interactions with the capsid proteins. Localized reconstruction is expected to provide novel biological insights in a range of challenging biological systems.
PDB ID: 5FJ5Download
MMDB ID: 133976
PDB Deposition Date: 2015/10/6
Updated in MMDB: 2015/11
Experimental Method:
electron microscopy
Resolution: 4.8  Å
Source Organism:
Similar Structures:
Biological Unit for 5FJ5: 120-meric
Molecular Components in 5FJ5
Label Count Molecule
Proteins (120 molecules)
Major Inner Protein P1(Gene symbol: phi-6L_4)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

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