5FIL: Bovine Mitochondrial Atp Synthase State 3b

Citation:
Abstract
Adenosine triphosphate (ATP), the chemical energy currency of biology, is synthesized in eukaryotic cells primarily by the mitochondrial ATP synthase. ATP synthases operate by a rotary catalytic mechanism where proton translocation through the membrane-inserted FO region is coupled to ATP synthesis in the catalytic F1 region via rotation of a central rotor subcomplex. We report here single particle electron cryomicroscopy (cryo-EM) analysis of the bovine mitochondrial ATP synthase. Combining cryo-EM data with bioinformatic analysis allowed us to determine the fold of the a subunit, suggesting a proton translocation path through the FO region that involves both the a and b subunits. 3D classification of images revealed seven distinct states of the enzyme that show different modes of bending and twisting in the intact ATP synthase. Rotational fluctuations of the c8-ring within the FO region support a Brownian ratchet mechanism for proton-translocation-driven rotation in ATP synthases.
PDB ID: 5FILDownload
MMDB ID: 133414
PDB Deposition Date: 2015/9/28
Updated in MMDB: 2016/12
Experimental Method:
electron microscopy
Resolution: 7.1  Å
Source Organism:
Similar Structures:
Biological Unit for 5FIL: 22-meric; determined by author and by software (PISA)
Molecular Components in 5FIL
Label Count Molecule
Proteins (22 molecules)
3
ATP Synthase Subunit Alpha, Mitochondrial(Gene symbol: ATP5F1A)
Molecule annotation
3
ATP Synthase Subunit Beta, Mitochondrial(Gene symbol: ATP5F1B)
Molecule annotation
1
ATP Synthase Subunit Gamma, Mitochondrial(Gene symbol: ATP5F1C)
Molecule annotation
1
ATP Synthase Subunit Delta, Mitochondrial(Gene symbol: ATP5F1D)
Molecule annotation
1
ATP Synthase Subunit Epsilon, Mitochondrial(Gene symbol: ATP5F1E)
Molecule annotation
8
ATP Synthase F(0) Complex Subunit C1, Mitochondrial(Gene symbol: ATP5MC1)
Molecule annotation
1
ATP Synthase Subunit O, Mitochondrial(Gene symbol: ATP5PO)
Molecule annotation
1
ATP Synthase F(0) Complex Subunit B1, Mitochondrial(Gene symbol: ATP5PB)
Molecule annotation
1
ATP Synthase Subunit D, Mitochondrial(Gene symbol: ATP5PD)
Molecule annotation
1
ATP Synthase-coupling Factor 6, Mitochondrial(Gene symbol: ATP5PF)
Molecule annotation
1
ATP Synthase Subunit Beta, Mitochondrial(Gene symbol: ATP6)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
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