5FB6: Room-temperature Macromolecular Crystallography Using A Micro- Patterned Silicon Chip With Minimal Background Scattering

Citation:
Abstract
Recent success at X-ray free-electron lasers has led to serial crystallography experiments staging a comeback at synchrotron sources as well. With crystal lifetimes typically in the millisecond range and the latest-generation detector technologies with high framing rates up to 1 kHz, fast sample exchange has become the bottleneck for such experiments. A micro-patterned chip has been developed from single-crystalline silicon, which acts as a sample holder for up to several thousand microcrystals at a very low background level. The crystals can be easily loaded onto the chip and excess mother liquor can be efficiently removed. Dehydration of the crystals is prevented by keeping them in a stream of humidified air during data collection. Further sealing of the sample holder, for example with Kapton, is not required. Room-temperature data collection from insulin crystals loaded onto the chip proves the applicability of the chip for macromolecular crystallography. Subsequent structure refinements reveal no radiation-damage-induced structural changes for insulin crystals up to a dose of 565.6 kGy, even though the total diffraction power of the crystals has on average decreased to 19.1% of its initial value for the same dose. A decay of the diffracting power by half is observed for a dose of D 1/2 = 147.5 +/- 19.1 kGy, which is about 1/300 of the dose before crystals show a similar decay at cryogenic temperatures.
PDB ID: 5FB6Download
MMDB ID: 140099
PDB Deposition Date: 2015/12/14
Updated in MMDB: 2016/06
Experimental Method:
x-ray diffraction
Resolution: 1.9  Å
Source Organism:
Similar Structures:
Biological Unit for 5FB6: tetrameric; determined by author and by software (PISA)
Molecular Components in 5FB6
Label Count Molecule
Proteins (4 molecules)
2
Insulin Chain a(Gene symbol: INS)
Molecule annotation
2
Insulin Chain B(Gene symbol: INS)
Molecule annotation
* Click molecule labels to explore molecular sequence information.

Citing MMDB
.